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1

Digitale Medien

The role of vitamin D in the medullary bone formation in egg-laying japanese quail and in immature male chicks treated with sex hormones (1983)

Takahashi, Naoyuki ; Shinki, Toshimasa ; Abe, Etsuko ; [weitere]

Springer

Calcified tissue international 35 (1983), S. 465-471

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ISSN: 1432-0827

Schlagwort(e): Medullary bone ; Mineralization ; Vitamin D3 ; Sex hormone

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Physik

Notizen: Summary The effect of vitamin D3 on medullary bone formation was investigated in egg-laying Japanese quail and in immature male chicks treated with sex hormones. When laying quail were fed a vitamin D-deficient diet for 16 days, their eggshell weights and egg production rate were markedly reduced in a time-dependent manner with a significant decrease in plasma calcium and 25-hydroxyvitamin D3 levels. The calcium content of the medullary bone of femurs decreased markedly with the progress of vitamin D deficiency, whereas that of the cortical bone remained unchanged. Quantitative histological examination also showed that the area of the mineralized portion of medullary bone in quail that were fed the vitamin D-deficient diet markedly decreased compared with that in the control laying quail, whereas the total area of the mineralized and unmineralized portions of medullary bone in the bone marrow cavity increased moderately. Daily administration of vitamin D3 (0.75 µg/day) to the vitamin D-deficient quail increased the mineralization of medullary bone as early as day 4. Daily administration of both estradiol (0.3 mg/day) and testosterone (0.9 mg/day) for 3 weeks to immature male chicks induced an apparent hypercalcemia and matrix formation of medullary bone, regardless of the vitamin D status of the chicks. Mineralization of medullary bone was observed only when vitamin D3 was administered together with the sex hormones. These results suggest that vitamin D3 is directly involved in the mineralization of medullary bone in birds.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02405078

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2

Digitale Medien

The role of vitamin D metabolites in the treatment of osteoporosis (1995)

Civitelli, Roberto ; Ogata, Eturo ; Avioli, Louis V. ; [weitere]

Springer

Calcified tissue international 57 (1995), S. 409-414

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ISSN: 1432-0827

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00301941

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3

Digitale Medien

Crystallinity and Superconducting Properties of YBa2Cu3O7−x Thin Films on NdGaO3 Substrate Prepared by Mist Microwave-plasma CVD (1998)

Takahashi, Naoyuki ; Koukitu, Akinori ; Seki, Hisashi

Springer

Journal of materials science 17 (1998), S. 877-879

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ISSN: 1573-4811

Quelle: Springer Online Journal Archives 1860-2000

Thema: Maschinenbau

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1006671429982

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4

Digitale Medien

Cloning of an osteoblastic cell line involved in the formation of osteoclast-like cells (1990)

Yamashita, Takeyoshi ; Asano, Katsuhiko ; Takahashi, Naoyuki ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 145 (1990), S. 587-595

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Experiments have been carried out to determine the mechanisms involved in the formation of osteoclast-like cells from spleen cells in mice. Osteoclasts were defined as tartrate-resistan. acid phosphatase-positive multinucleated cells (TRACP-positive MNCs) in which specific calcitonin receptors were identified by autdradiography with labeled salmon calcitonin. Furthermore, cultures rich in these cells produced resorption pits when grown on dentine slices. Several clonal cell lines were obtained from fetal mouse calvariae and screened for their ability to induce TRACP-positive MNCs in response to 1α, 25-dihydroxyvitamin D3 “1α,25(OH)2D3” in co-cultures with spleen cells. A cell line, KS-4, was identified with the greatest potency in inducing osteolast-like cell formation in co-culture with spleen cells. The capacity of KS-4 cells to produce this effect was much greater than that of two bone marrow-derived stromal cell lines (MC3T3-G2/PA6 and ST2 cells), which we have previously shown to be effective in this system but to require treatment with dexamethasone in addition to 1α25(OH)2D3 (Udagawa et al.: Endocrinology 125:1805-1813, 1989). Parathyroid hormone (PTH) in creased cAMP production in KS-4 cells, and PTH and interleukin-1α also induced TRACP-positive MNCs in co-cultures with spleen cells. Contact between living KS-4 and spleen cells was necessary for osteoclast formation to take place, since this did not occur when the two populations were separated by a membrane filter, or when the KS-4 cells were killed by fixation. Separate cultures of either spleen cells or KS-4 cells formed no TRACP-positive MNCs. KS-4 cells synthesized predominantly type I collagen, formed bone nodules without added of β-glycerophosphate in a long-term culture, and expressed increasing alkaline phosphatase activity after confluence in culture. These results indicate that the KS-4 cells have properties consistent with progression toward the osteoblast phenotype, and represent a single cell line with the ability to promote osteoclast formation by a contact-requiring process.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041450327

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5

Digitale Medien

Effect of a continuously applied compressive pressure on mouse osteoblast-like cells (MC3T3-E1) in vitro (1990)

Ozawa, Hiroyuki ; Imamura, Kazunobu ; Abe, Etsuko ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 142 (1990), S. 177-185

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Bone metabolism is often affected by a variety of mechanical forces, but the cytological basis of their action is not known. In this study, we examined the effect of a continuously applied compressive pressure (CCP) on the growth and differentiation of clonal mouse osteoblast-like cells (MC3T3-E1) cultured in a specifically devised culture chamber. The gas phase of the chamber was maintained at a pressure of 2 atmospheres (atm) above ambient (3 atm total, 3.1 kg/cm2; 3.0 × 105 Pa) by continuously infusing a compressed mixed gas (O2: N2:CO2 = 7.0%:91.3%:1.7%). The pO2, pCO2, and pH in the culture medium at 37°C under 3 atm were maintained at the same levels as those under 1 atm. MC3T3-E1 cells were cultured in α minimal essential medium containing 10% fetal bovine serum under either 3 atm in the CCP culture chamber or 1 atm in an ordinary CO2 incubator. Alkaline phosphatase activity, a marker of osteoblasts, was greatly suppressed by the CCP treatment. The inhibition of alkaline phosphatase activity was rapidly restored when the cells were transferred to an ordinary CO2, incubator under 1 atm, indicating that the inhibition of alkaline phosphatase activity by CCP is reversible. Cell growth was not altered under CCP. The CCP treatment greatly increased the production and secretion of prostaglandin E2(PGE2). Adding either conditioned medium from the CCP culture or exogenous PGE2 to the control culture under 1 atm suppressed alkaline phosphatase activity dose-dependently. The CCP treatment also suppressed collagen synthesis and calcification. These results suggest that CCP causes the cells to produce and secrete PGE2 which, in turn, inhibits differentiation of osteoblasts and the concomitant calcification.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041420122

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6

Digitale Medien

Continuously applied compressive pressure induces bone resorption by a mechanism involving prostaglandin E2 synthesis (1990)

Imamura, Kazunobu ; Ozawa, Hiroyuki ; Hiraide, Takatoshi ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 144 (1990), S. 222-228

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: In previous research, we devised a specific culture chamber to examine the effect of continuously applied compressive pressure (CCP) on bone formation and resorption. The chamber was infused with compressed mixed gases with different O2 and CO2 composition to maintain the pO2, pCO2, and pH in the culture medium under pressures of +0.5 atm (1.5 atm total) to +2.0 atm (3.0 atm total) at the same levels as those at the ordinary pressure (1 atm). Using the specific culture chamber, we demonstrated that CCP greatly suppressed the differentiation of mouse osteoblast-like MC3T3-E1 cells. The inhibition by CCP appeared to be mediated by prostaglandin E2 (PGE2). In the present study, we examined the effect of CCP on osteoclastic bone resorption. CCP treatment of mouse bone marrow culture markedly increased both the PGE2 production and the number of tartrateresistant acid phosphatase (TRACP)-positive mononuclear cells (possibly precursors of multinucleated osteoclasts). An autoradiographic study using [125l]-salmon calcitonin showed clearly that those TRACP-positive cells had calcitonin receptors. The CCP effect was the greatest at + 1.0 atm (2.0 atm total). Isobutylmethylxanthine potentiated the production of TRACP-positive cells induced by CCP. Adding indomethacin completely inhibited both the TRACP-positive cell formation and the PGE2 production induced by CCP. CCP also increased the release of 45Ca from prelabeled mouse calvaria during later stages (2-6 days) of the 6-day culture period. CCP markedly increased PGE2 but not interleukin 1 in the culture media of mouse calvaria. These results indicate that, besides inhibiting osteoblast differentiation, CCP stimulates bone resorption by generating new osteoclasts through a mechanism involving PGE2 production.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041440207

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7

Digitale Medien

Role of vitamin D in bone resorption (1992)

Suda, Tatsuo ; Takahashi, Naoyuki ; Abe, Etsuko

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 49 (1992), S. 53-58

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ISSN: 0730-2312

Schlagwort(e): bone mineralization ; anti-rachitic agent ; osteoclast ; osteoblast ; mononuclear phagocytes ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: The idea that vitamin D must function at the bone site to promote bone mineralization has long existed since its discovery as an anti-rachitic agent. However, the definite evidence for this is still lacking. In contrast, much evidence has accumulated that 1α,25(OH)2D3 is involved in bone resorption. 1α,25(OH)2D3 tightly regulates differentiation of osteoclast progenitors into osteoclasts. Osteoclast progenitors have been thought to belong to the monocyte-macrophage lineage. 1α,25(OH)2D3 greatly stimulates differentiation and activation of mononuclear phagocytes. Recent reports have indicated that differentiation of mononuclear phagocytes into osteoclasts is strictly regulated by osteoblastic cells, the process of which is also stimulated by 1α,25(OH)2D3. In the differentiation of mononuclear phagocytes into osteoclasts, the target cells for 1α,25(OH)2D3 appear to be osteoblastic stromal cells. Osteoblastic cells produce several proteins such as BGP, MGP, osteopontin and the third component of complement (C3) in response to the vitamin. They appear to be somehow involved in osteoclast differentiation and functions. Thus, 1α,25(OH)2D3 seems to be involved in the differentiation of osteoclast progenitors into osteoclasts directly and also by an indirect mechanism involving osteoblastic cells. The precise role of osteoblastic cells in osteoclast development has to be elucidated in the future.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240490110

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8

Digitale Medien

1α,25-Dihydroxyvitamin D3 modulation in lipid metabolism in established bone marrow-derived stromal cells, MC3T3-G2/PA6 (1992)

Shionome, Manabu ; Shinki, Toshimasa ; Takahashi, Naoyuki ; [weitere]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 48 (1992), S. 424-430

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ISSN: 0730-2312

Schlagwort(e): adipocyte ; adipogenesis ; osteoporosis ; phospholipids ; preadipocyte ; triacylglycerol ; vitamin D derivatives ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: MC3T3-G2/PA6 (PA6) cells established from newborn mouse calvaria are preadipocytic stromal cells, which differentiate into adipocytes in response to glucocorticoids. We examined the effects of 1α,25-dihydroxyvitamin D3[1α,25(OH)2D3] on adipogenesis in PA6 cells were cultured with 10-8 M dexamethasone, adipocytes containing oil red O-positive droplets first appeared on day 7 (3 days after confluence was attained) and the maximal synthesis of neutral lipids occurred on day 12. Simultaneous addition of 1α,25(OH)2D3 at 10-9 M completely blocked this dexamethasone-induced neutral lipid synthesis throughout the 14-day culture period. Dose-response studies of vitamin D3 derivatives showed that 1α,25(OH)2D3 was the most potent in inhibiting neutral lipid synthesis in PA6 cells, followed by 1α-hydroxyvitamin D3, 25-hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3, in that order. Dexamethasone greatly enhanced incorporation of [14C]-acetic acid into triacylglycerol in PA6 cells. The incorporation was markedly inhibited by the addition of 10-9 M 1α,25(OH)2D3 Instead, 1α,25(QH)2D3 greatly increased incorporation of [14C]-acetic acid into phospholipids, such as phosphatidylcholine and phosphatidylethanolamine, irrespective of the presence or absence of dexamethasone. These results suggest that 1α,25(OH)2D3 modulation of lipid metabolism in bone marrow stromal cells is receptor mediated.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240480411

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9

Digitale Medien

Possible involvement of focal adhesion kinase, p125FAK, in osteoclastic bone resorption (1995)

Tanaka, Sakae ; Takahashi, Naoyuki ; Udagawa, Nobuyuki ; [weitere]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 58 (1995), S. 424-435

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ISSN: 0730-2312

Schlagwort(e): Key words ; osteoclast ; focal adhesion kinase ; tyrosine kinase ; tyrosine phosphorylation ; podosome ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Involvement of tyrosine phosphorylation in osteoclastic bone resorption was examined using osteoclast-like multinucleated cells prepared from co-cultures of mouse osteoblastic cells and bone marrow cells in the presence of 1α,25-dihydroxyvitamin D3. When osteoclast-like cells were plated on culture dishes in the presence of 10% fetal bovine serum, they were sharply stained in their peripheral region by anti-phosphotyrosine antibody. Western blot analysis revealed that 115-to 130-kD proteins were tyrosine-phosphorylated in osteoclast-like cells. Using immunoprecipitation and immunoblotting, one of the proteins with 115-130 kD was identified as focal adhesion kinase (p125FAK), a tyrosine kinase, which is localized in focal adhesions. Immunostaining with anti-p 125FAK antibody revealed that p125FAK was mainly localized at the periphery of osteoclast-like cells. Herbimycin A, a tyrosine kinase inhibitor, not only suppressed tyrosine phosphorylation of p125FAK but also changed the intracellular localization of p125FAK and disrupted a ringed structure of F-actin-containing podosomes in osteoclast-like cells. Antisense oligodeoxynucleotides to p125FAK inhibited dentine resorption by osteoclast-like cells, whereas sense oligodeoxynucleotides did not. These results suggest that p125FAK is involved in osteoclastic bone resorption and that tyrosine phosphorylation of p125FAK is critical for regulating osteoclast function.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcb.240580405

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