Publication Date:
1991-11-15
Description:
Crystallization of macromolecules for structural studies has long been a hit-or-miss process. The crystallization of hexanucleotides as Z-DNA was studied, and it was shown that the cation concentration for crystal formation could be predicted from solvation free energy (SFE) calculations. Solution studies on the conformation and solubilities of the hexanucleotides showed that a critical concentration of the DNA in the Z-conformation must be present in solution to effect crystallization. The SFE calculations therefore predict the propensity of the hexanucleotides to adopt the left-handed conformation and the driving force required to reach this critical concentration relative to the intrinsic solubility of Z-DNA for crystallization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ho, P S -- Kagawa, T F -- Tseng, K H -- Schroth, G P -- Zhou, G W -- New York, N.Y. -- Science. 1991 Nov 15;254(5034):1003-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1948069" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Cations
;
Crystallography
;
DNA/*chemistry/ultrastructure
;
Models, Molecular
;
Nucleic Acid Conformation
;
Oligodeoxyribonucleotides/*chemistry
;
Spectrophotometry, Ultraviolet
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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