Publication Date:
2009-01-13
Description:
Recurrent gene fusions, typically associated with haematological malignancies and rare bone and soft-tissue tumours, have recently been described in common solid tumours. Here we use an integrative analysis of high-throughput long- and short-read transcriptome sequencing of cancer cells to discover novel gene fusions. As a proof of concept, we successfully used integrative transcriptome sequencing to 're-discover' the BCR-ABL1 (ref. 10) gene fusion in a chronic myelogenous leukaemia cell line and the TMPRSS2-ERG gene fusion in a prostate cancer cell line and tissues. Additionally, we nominated, and experimentally validated, novel gene fusions resulting in chimaeric transcripts in cancer cell lines and tumours. Taken together, this study establishes a robust pipeline for the discovery of novel gene chimaeras using high-throughput sequencing, opening up an important class of cancer-related mutations for comprehensive characterization.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725402/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725402/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maher, Christopher A -- Kumar-Sinha, Chandan -- Cao, Xuhong -- Kalyana-Sundaram, Shanker -- Han, Bo -- Jing, Xiaojun -- Sam, Lee -- Barrette, Terrence -- Palanisamy, Nallasivam -- Chinnaiyan, Arul M -- R01 CA132874/CA/NCI NIH HHS/ -- R01 CA132874-01A1/CA/NCI NIH HHS/ -- U01 CA111275/CA/NCI NIH HHS/ -- U01 CA111275-04/CA/NCI NIH HHS/ -- U54 DA 021519/DA/NIDA NIH HHS/ -- England -- Nature. 2009 Mar 5;458(7234):97-101. doi: 10.1038/nature07638. Epub 2009 Jan 11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Michigan Center for Translational Pathology, Ann Arbor, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19136943" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Cell Line, Tumor
;
Fusion Proteins, bcr-abl/analysis/genetics
;
Gene Expression Profiling/*methods
;
Humans
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics
;
Male
;
Molecular Sequence Data
;
Neoplasms/*genetics
;
Oncogene Proteins, Fusion/*analysis/*genetics
;
Prostatic Neoplasms/genetics
;
Sequence Analysis, DNA/instrumentation/*methods
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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