ISSN:
1573-4943
Keywords:
Trypsin inhibitor
;
Kunitz-type seed inhibitor
;
amino acid sequence
;
sequence similarity
;
Leguminosae
;
winged bean
;
Psophocarpus tetragonolobus (L.) DC
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The primary structure of acidic trypsin inhibitor-2a (WBTI-2a,pI 5.9) fromPsophocarpus tetragonolobus (L.) DC seed was determined. This inhibitor consists of a single polypeptide chain of 180 amino acids including four half-cystine residues and has an N-terminal residue of pyroglutamic acid. The sequence of WBTI-2a,pI 5.9, showed 84% identity to acidic trypsin inhibitor-2 (WBTI-2,pI 5.1) but only 57% identity to the basic trypsin inhibitor (WBTI-1,pI 8.9) and 50% identity to the chymotrypsin inhibitor of winged bean. The data indicate that winged bean seed contains a family of three Kunitz-type inhibitors which have about 50% identity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01024782
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