ISSN:
1573-8221
Keywords:
Spleen
;
continuous culture
;
“shaking” method
;
phytohemagglutinin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A continuous line of human spleen cells was obtained. Important factors in the isolation of this strain were seeding a large number of cells and prolonged incubation of the culture; these two factors created conditions for gradual modification of cellular metabolism, without which the cells could not have adapted themselves to prolonged culture. A continuous culture could not be obtained from one colony and a “shaking” method had therefore to be used. Cultures were obtained only after the addition of 1% phytohemagglutinin to the nutrient medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00785571
Permalink