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  • 1
    Electronic Resource
    Electronic Resource
    Fabrication, Surface Modification and Analysis of Biocompatibility of Biologic Chitosan Scaffold (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 373-374 (Mar. 2008), p. 654-657 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this study, the surface modification and biocompatibility of the biologic chitosanscaffold were investigated. The chitosan scaffold with excellent reticular structure was attained afterbeing purified, emulsionized, cross-linked, molded and freeze-dried step by step by using the nativematerials, coming from such as lobster shell, crab shell etc.. After that, its surface modification wasoperated with film coating by using gelatin. Then the bone marrow mesenchymal stem cells (BMSCs)derived from New Zealand rabbits were used as the seed cells and were inoculated onto the modifiedbiologic chitosan scaffolds at 3×105 cells/ml to investigate the biocompatibility and bone conductiveefficiency of this kind of scaffold in static culture for one week. As a control, the cell suspensions withsame densities were inoculated onto the chitosan scaffold without being treated. During the wholeculture process, the cellular adherence and expansion were observed under inverted microscope.After culture, the biological properties of the fabricated cell-scaffold tissues were detected byscanning electron microscope (SEM) and HO/PI fluorescent double staining. The results showed thatthe biologic chitosan scaffold treated with gelatin or rat-tail collagen promoted a higher adhesion andproliferation of BMSCs in comparison to the untreated samples. Besides, the BMSCs within thetreated scaffold were more regular and well-distributed than those in untreated one. It is concludedthat this kind of surface modification can be used to change the physicochemical properties ofchitosan scaffold. The improved biologic chitosan scaffold is suitable to be an ideal biomedicalscaffold for tissue engineering
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.654.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Bio-Derived Bone Surface Modification with Biomimetic Thin Film Coatings to Improve Mesenchymal Stem Cells Adhesion and Spreading (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 373-374 (Mar. 2008), p. 722-725 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Initial attachment and spreading of the inoculated cells determines the long-time viabilityof cells onto biomedical scaffolds designed for various orthopedic or other clinical applications. Theaim of this study was to investigate the influence of biomimetic thin film coating surfaces ofbio-derived bone scaffolds with collagen proteins and chitosan on bone marrow mesenchymal stemcells interactions in order to improve cell adhesion, spreading and proliferation. These two meritswere used synthetically to generate apatite-based materials that can function as allograft bone graftsin humans. In this study, the thin film coatings were operated by means of soaked, pre-frozen, andfreeze-dried step by step. All coatings were characterized using Raman spectra, inverted microscope,atomic force microscopy, and scanning electron microscopy. After that, the bio-derived bonescaffolds with or without thin film coatings were used in bone marrow mesenchymal stem cell cultureexperiments to study cell adhesion, spreading, viability, proliferation and morphology. Then, thebiological morphologies of the fabricated cell-scaffold constructs were detected by scanning electronmicroscope (SEM). The cell reactions were investigated concerning cell adhesion, migration,spreading, and proliferation under inverted microscope and fluorescence microscopy. The resultsshowed that the bio-derived bone scaffold treated with thin film coatings by using rat-tail type Icollagen and chitosan improved the adhesion and spreading of mesenchymal stem cells incomparison to the untreated one. Besides, cell viability and morphology were not affected by thepresence of either type of thin film coating. Still, the results assay revealed an increased proliferationof bone marrow mesenchymal stem cells on both types of thin film coatings compared to coating withnon-coated controls
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.722.pdf
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