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  • 1
    Electronic Resource
    Electronic Resource
    Adsorption of DNA on clay minerals: protection against DNaseI and influence on gene transfer (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 97 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Numerous authors have investigated DNA relationships with sandy soil. A model composed of various DNAs adsorbed on montmorillonite clay was developed to assay enzyme (DNaseI) activity on clay-adsorbed nucleic acids. The extent of DNA adsorption was affected by the concentration and valency of the cations used (Mg2+, Ca2+, Na+), indicating a charge-dependent process. Calf thymus DNA was found to be highly adsorbed by smectite (up to 30 mg g−1 of dry clay). Adsorbed DNA was shown to be more resistant to degradation by DNaseI than free DNA. Experimental data with plasmid and short linear amplified (through polymerase chain reaction) DNA showed that protection against nucleases was only partial. Nevertheless, clay-adsorbed DNA was found to be still able, even after a strong DNaseI treatment, to artificially transform competent Escherichia coli cells. The results show that persistance of DNA and gene transfer by genetic transformation may occur in soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05435.x
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  • 2
    Electronic Resource
    Electronic Resource
    Formation and regeneration of Frankia protoplasts (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 70 (1987), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A technique for forming protoplasts from Frankia cells and regenerating them to the normal hyphal mode of growth is described. Electron microscopy proved that protoplasts were studied and not spores or small hyphae. Regenerated colonies were investigated for genetic markers. One ArI3 colony had been cured of its plasmids without being affected in its symbiotic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1987.tb06142.x
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  • 3
    Electronic Resource
    Electronic Resource
    Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 21 (1996), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The aim of this work was to determine the efficiency of the conjugative plasmid pTS130 to transfer in various environmental conditions between two strains of Streptomyces lividans. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofaciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces lividans cells or the transfer rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1996.tb00343.x
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  • 4
    Electronic Resource
    Electronic Resource
    On the track of natural transformation in soil (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 15 (1994), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The understanding of microbial gene transfer including how bacteria acquire and disseminate genes in natural environments will provide data on the role of horizontal transfer in evolution. This understanding has been stimulated in recent years by concern about the impact of genetically engineered microorganisms on natural environments. This prospect has increased interest in determining the regulatory mechanisms of indigenous microbial populations as well as detecting genetic interactions between bacteria introduced into soil and the indigenous microflora. This paper will review the strategies developed to demonstrate whether the different steps required by natural bacterial transformation (the uptake of naked DNA by competent bacteria) could actually occur in soil. This will include a review on the release of DNA from microbial cells by passive or active mechanisms, its persistence by adsorption of extracellular DNA onto major soil components such as sand or clay minerals and the uptake of DNA by competent bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1994.tb00235.x
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  • 5
    Electronic Resource
    Electronic Resource
    Erratum to “Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil” (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 40 (2002), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2002.tb00939.x
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  • 6
    Electronic Resource
    Electronic Resource
    Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 38 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In Streptomyces lividans, a conditional lethal mechanism was obtained by inactivation of gylB in the glycerol operon. The resulting strain was efficiently counter-selected on glycerol-containing medium but the mutation had no effect on the capacity of the strain to be maintained in non-sterile soil samples and to transfer derivatives of the genetic element pSAM2. However, despite the efficiency of the donor counter-selection system, the transfer of a pSAM2 derivative from S. lividans to indigenous soil bacteria was not detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00889.x
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  • 7
    Electronic Resource
    Electronic Resource
    Heterologous hybridization of Frankia DNA to Rhizobium meliloti and Klebsiella pneumoniae nif genes (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 55 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A nif gene probe from Rhizobium meliloti was used to isolate a recombinant bacteriophage from a Frankia sp. ArI3 gene bank. There is a large homology between nifD and nifH genes of R. meliloti or Klebsiella pneumoniae and Frankia DNA sequences. Approximately 4.5 kb to the right of nifK, we have localized a DNA region hybridizing to a R. meliloti probe containing nifA and nifB genes. The extent of the homology was greater for nifB than for nifA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb13923.x
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  • 8
    Electronic Resource
    Electronic Resource
    Presence of a linear plasmid-like DNA molecule in the fungal pathogen Ceratocystis fimbriata Ell. & Halst. (1987)
    Springer
    Current genetics 11 (1987), S. 335-338 
    Details
    ISSN: 1432-0983
    Keywords: Ceratocystis fimbriata ; Linear plasmid ; mtDNA ; Poplar pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A plasmid-like molecule was detected in a strain of the ascomycete Ceratocystis fimbriata Ell. & Halst., a pathogenic fungus of Populus spp. The DNA replicon, designated pFQ501, was found to have a linear structure with a length of 6.0 kb (3.9 × 106 daltons) and a density of 1.685 g/cc. This molecule was found to be associated with the mitochondria and was isolated from the gel; its restriction map was deduced from single and double digestions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00355410
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer (1992)
    Springer
    Archives of microbiology 157 (1992), S. 107-115 
    Details
    ISSN: 1432-072X
    Keywords: Nitrobacter ; Nitrification ; Natural population ; soil ; freshwater ; sediments ; Restriction fragment length polymorphism ; Polymerase chain reaction ; Intergenic spacer ; ribosomal RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequences from the intergenic spacer (IGS) region of the ribosomal operon were amplified by the polymerase chain reaction (PCR) technique using two primers derived from 16S and 23S rRNA conserved sequences. The PCR products, cleaved by 4 base cutting restriction enzymes, were used to differentiate Nitrobacter strains. This method offered a convenient alternative to serological testing for characterization of Nitrobacter isolates and enabled a large number of strains to be genotypically characterized easily and rapidly. This method was successfully used to characterize natural populations of Nitrobacter from various soils and a lake. A diversity was demonstrated in various soils, and in a lake both in freshwater and in sediments. Strains closely related to both WL and LL were found in these eco-systems. It seems that the diversity of Nitrobacter populations was not associated with global environments but may be related to the presence of locally coexisting niches.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245277
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  • 10
    Electronic Resource
    Electronic Resource
    Conservation of nif sequences in Frankia (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 238-246 
    Details
    ISSN: 1617-4623
    Keywords: Frankia ; Nitrogen fixation ; Alnus ; Symbiosis ; nifH nucleotide sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Southern blots of Frankia total DNAs were hybridized with nifHDK probes from Rhizobium meliloti, Klebsiella pneumoniae and Frankia strain Arl3. Differences between strains were noted in the size of the hybridizing restriction fragments. These differences were more pronounced among Elaeagnus-compatible strains than among Alnus- or Casuarina-compatible strains. Gene banks constructed for Frankia strains EUN1f, HRN18a, CeD and ACoN24d were used to isolate nif-hybridizing restriction fragments for subsequent mapping and comparisons. The nifH zone had the highest sequence conservation and the nifH and nifD genes were found to be contiguous. The complete nucleotide sequence of the nifH open reading frame (ORF) from Frankia strain Arl3 is 861 bp in length and encodes a polypeptide of 287 amino acids. Comparisons of these nucleic acid and amino acid sequences with other published nifH sequences suggest that Frankia is most similar to Anabaena and Azotobacter spp. and K. pneunoniae and least similar to the Gram-positive Clostridium pasteurianum and to the archaebacterium Methanococcus voltae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339587
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