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  • 1
    Electronic Resource
    Electronic Resource
    Studies on analgesic agents. 1. Preparation of 1,2-diphenyl-2-(4-substituted 1-piperazinyl)ethanol derivatives and structure-activity relationships (1979)
    s.l. : American Chemical Society
    Journal of medicinal chemistry 22 (1979), S. 58-63 
    Details
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jm00187a014
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of bone protein components with polyacrylamide gel electrophoresis: effects of zinc and hormones in tissue culture (1992)
    Springer
    Molecular and cellular biochemistry 117 (1992), S. 153-158 
    Details
    ISSN: 1573-4919
    Keywords: bone protein ; zinc ; estrogen ; insulin ; rat calvaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract An attempt was made to clarify the molecular characterization of zinc-induced bone protein synthesis in tissue culture. Calvaria were removed from weanling rat (3-week-old male) and cultured for periods up to 48 hr in Dulbecco's Modified Eagle Medium (high Glucose, 4500 mg/dl) supplemented with antibiotics and bovine serum albumin. When calvaria cultured in the presence of 10−5 to 10−4 M zinc were pulsed with [3H] leucine, zinc caused a significant increase in the incorporation of [3H] leucine into the acid-insoluble residues of bone tissue. The soluble fraction obtained from cultured bone was analyzed with SDS-polyacrylamide gel electrophoresis (SIDS-PAGE). The major components in the fraction obtained from control bone were 68 killo-dalton (kDa) and 45 kDa proteins. These components were clearly increased by the presence of zinc (10−4 M). The effect of zinc was completely abolished by the coexistence of 10−6 M cycloheximide. Meanwhile, 10−9 M estrogen or 10−8 M insulin, which can stimulate bone formation, did not enhance the effect of zinc to increase bone 68 and 45 kDa proteins. The present findings suggest that zinc increases many bone protein components, especially 68 and 45 kDa proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230754
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of calcium-binding protein regucalcin mRNA in rat liver is stimulated by calcitonin: The hormonal effect is mediated through calcium (1994)
    Springer
    Molecular and cellular biochemistry 136 (1994), S. 43-48 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; rat liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The involvement of a hypocalcemic hormone calcitonin (CT) in the expression of hepatic Ca2+-binding protein regucalcin mRNA was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb). A single oral administration of calcium chloride (100 mg Ca/100 g body weight) to rats induced a remarkable increase in the serum calcium concentration and a corresponding elevation of the liver calcium content during 120 min after the administration. Thyroparathyroidectomy (TPTX) did not cause a significant increase in the liver calcium content after calcium administration. Hepatic regucalcin mRNA level was markedly elevated by calcium administration; the level was about 180% of controls at 60 min after the administration. This increase was completely abolished by TPTX. A single subcutaneous administration of CT (synthetic eel CT; 25–100 MRC mU/100 g) to TPTX rats received oral administration of calcium (100 mg/100 g) produced a remarkable increase in hepatic regucalcin mRNA levels; the level was about 280% of controls with the dose of 25 MRC mU CT/100 g. The present finding suggests that the expression of hepatic mRNA is stimulated by CT, and that the hormonal effect is mediated through Ca2+ in rat liver.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931603
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of hepatic calcium-binding protein regucalcin mRNA is decreased by phenobarbital administration in rats (1994)
    Springer
    Molecular and cellular biochemistry 141 (1994), S. 15-19 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; phenobarbital ; rat liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of phenobarbital on the expression of calcium-binding protein regucalcin mRNA in rat liver was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.9 kb of open reading frame). Phenobarbital (4, 8 and 12 mg/ 100 g body weight) was intraperitoneally administered to rats 3 times with 24 h intervals, and the animals were sacrificed by bleeding at 24 h after the last administration. The hepatic regucalcin mRNA levels were markedly reduced by phenobarbital administration. This decrease was about 50% of control level with the 12 mg/100 g dose. Moreover, the hepatic regucalcin concentration was significantly decreased by the administration of phenobarbital (12 mg/100 g), although the serum regucalcin concentration was not altered appreciably. Meanwhile, serum transaminases (GOT and GPT) activities were not increased by the administration of phenobarbital (4 and 12 mg/100 g). The present study demonstrates that the expression of hepatic regucalcin mRNA is decreased by phenobarbital administration in rats, suggesting that regucalcin does not have a role in drug metabolism related to phenobarbital.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935586
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  • 5
    Electronic Resource
    Electronic Resource
    Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin (1995)
    Springer
    Molecular and cellular biochemistry 143 (1995), S. 67-71 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; gene distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The existence and expression of gene encoding the Ca2+-binding protein regucalcin in various species and tissues were investigated with Southern and Northern hybridization analyses using regucalcin cDNA (0.9 kb of open reading frame). Genomic Southern hybridization analysis demonstrated that regucalcin gene was widely conserved among higher animals including human, monkey, rat, mouse, dog, bovine, rabbit and chicken. The gene was not found in yeast. The Northern blot analysis of poly (A)+RNAs extracted from the liver of various species showed that regucalcin mRNA was predominantly expressed in rat and mouse, although the expression was also seen in human, bovine and chicken. Furthermore, the enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG indicated that hepatic regucalcin concentration was most pronounced in rat as compared with that of guinea pig, mouse and chicken. These observations show that the gene expression of regucalcin and its protein synthesis is unique in the liver of rats, suggesting the existence of a specific mechanism in demonstrating regucalcin synthesis from gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00925928
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  • 6
    Electronic Resource
    Electronic Resource
    Genomic cloning and chromosomal assignment of rat regucalcin gene (1995)
    Springer
    Molecular and cellular biochemistry 151 (1995), S. 157-163 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene cloning ; FISH ; chromosomal localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The gene for a Ca2+-binding protein regucalcin was cloned from a rat genomic library which was constructed in λ FIX II by screening with radiolabeled probe (complementary DNA of rat liver regucalcin). Positive clone had 19.9 kb insert of size and contained four exons of the gene coding for a rat regucalcin. These exons included the partial coding sequence (61.2% of open reading frame) and the entire 3′-untranslated region of the gene. The nucleotide sequence of exons completely agreed with that of a rat regucalcin cDNA clone. The sequence analysis of the clone showed that the identifier sequence and two simple repeated sequences exist in the intron of the gene. Moreover, chromosomal location of the rat regucalcin gene was determined by direct R-banding fluorescencein situ hybridization (FISH) method with the 19.9 kb clone containing four exons. The regucalcin gene was localized on rat chromosome Xq11.1–12 proximal end.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322338
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  • 7
    Electronic Resource
    Electronic Resource
    Hepatic calcium-binding protein regucalcin is released into the serum of rats administered orally carbon tetrachloride (1994)
    Springer
    Molecular and cellular biochemistry 131 (1994), S. 173-179 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; liver injury ; carbon tetrachloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The change in calcium-binding protein regucalcin, mainly localized in liver, in the liver and serum of rats received a single oral administration of carbon tetrachloride (50%; 1.0 ml/100 g body weight) was investigated. The change of regucalcin mRNA levels in the liver was analyzed by Northern blotting using liver regucalcin cDNA (0.6 kb). At 10 and 24 h after the administration, liver regucalcin mRNA levels were reduced markedly. Moreover, regucalcin concentration in the liver and serum was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. Administration of carbon tetrachloride (CCl4) induced a significant decrease in liver regucalcin concentration and a corresponding elevation of serum regucalcin concentration at 24 h after the administration. An appreciable increase in serum regucalcin concentration was seen at 2 h after the administration. Meanwhile, serum transaminases (GOT and GPT) activities were significantly increased by CCl4 administration, indicating that liver injury is induced. The present study demonstrates that hepatic regucalcin is released into the serum of rats administered orally CCl4, suggesting that the estimation of serum regucalcin is a useful tool for diagnosis of liver injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00925954
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  • 8
    Electronic Resource
    Electronic Resource
    Cloning of MafG homologue from the rat brain by differential display and its expression after hypercapnic stimulation (2000)
    Springer
    Molecular and cellular biochemistry 203 (2000), S. 135-141 
    Details
    ISSN: 1573-4919
    Keywords: MafG ; nuclear transcription factor ; ventral medullary surface ; differential display ; tissue distribution ; H+-sensing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The ventral medullary surface (VMS) is a site of the medullary chemoreceptor neurons which sense excess protons (H+) derived from hypercapnia and facilitate respiration. We hypothesized that expression of genes involved in H+-sensitivity is higher in the VMS than in other central nervous system areas. By using the differential display technique, we differentiated the mRNAs of VMS neurons from those of cerebral cortical neurons. Seventeen clones of interest were isolated, and sequence analysis revealed that one of these clones had an encoding nuclear transcription factor, MafG. MafG is a member of Maf protein family, and the founding member of the family (v-Maf) was originally discovered as the transduced transforming component of avian musculoaponeuroticfibrosarcoma virus, AS42. The rat MafG was composed of 162 amino acid residues and was conserved among the primary structures of various species. Expression of rat mafG mRNA is high in the VMS, heart and skeletal muscle while the cerebral cortex, cerebellum, liver, stomach and intestine show moderate expression. To determine whether the expression of mafG mRNA is induced by hypercapnic stimulation, 7% CO2 in air was inhaled to rats for 5 min. We found that the hypercapnic stimulation induced the gene expression of mafG. These results suggest that MafG may be involved in H+-sensitivity and respiratory regulation in the VMS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007017902194
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  • 9
    Electronic Resource
    Electronic Resource
    The 5′ end sequences and exon organization in rat regucalcin gene (1996)
    Springer
    Molecular and cellular biochemistry 165 (1996), S. 145-150 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene cloning ; gene organization ; 5′ end sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The 5−flanking region of the gene for a Ca2−-binding protein regucalcin was cloned from a rat genomic library which was constructed in lambda EMBL3 SP6/T7 vector. The genomic library was screened by using the radiolabeled probe with the 5′ region (0.5 kb) of rat regucalcin complementary deoxyribonucleic acid (cDNA). Positive clone had the 5.5 kb fragment which was hybridized with the 5′-probe. This fragment contained three exons (I–III) of the gene coding for a rat regucalcin. The nucleotide sequence of exons completely agreed with that of a rat regucalcin cDNA clone. A supposed translational initiation site existed in the exon 11. Homology analysis showed that a putative transcription start site in the rat regucalcin gene was located at position 26 downstream from a TATA-box. Another upstream element, a CCAAT box-like sequence, was located at −170. Moreover, there were many regulatory elements (Hox, AP-1, AP-2 and AP-4) in the 5′-flanking region of the rat regucalcin gene. The organization of rat regucalcin gene seemed to be about 18 kb in size and consisted of seven exons and six introns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229476
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  • 10
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    Maternal prolactin during late pregnancy is important in generating nurturing behavior in the offspring (2017)
    National Academy of Sciences
    Details
    Publication Date: 2017-11-20
    Description: Although maternal nurturing behavior is extremely important for the preservation of a species, our knowledge of the biological underpinnings of these behaviors is insufficient. Here we show that the degree of a mother’s nurturing behavior is regulated by factors present during her own fetal development. We found that Cin85-deficient (Cin85−/−) mother mice had reduced pituitary hormone prolactin (PRL) secretion as a result of excessive dopamine signaling in the brain. Their offspring matured normally and produced their own pups; however, nurturing behaviors such as pup retrieval and nursing were strongly inhibited. Surprisingly, when WT embryos were transplanted into the fallopian tubes of Cin85−/− mice, they also exhibited inhibited nurturing behavior as adults. Conversely, when Cin85−/− embryos were transplanted into the fallopian tubes of WT mice, the resultant pups exhibited normal nurturing behaviors as adults. When PRL was administered to Cin85−/− mice during late pregnancy, a higher proportion of the resultant pups exhibited nurturing behaviors as adults. This correlates with our findings that neural circuitry associated with nurturing behaviors was less active in pups born to Cin85−/− mothers, but PRL administration to mothers restored neural activity to normal levels. These results suggest that the prenatal period is extremely important in determining the expression of nurturing behaviors in the subsequent generation, and that maternal PRL is one of the critical factors for expression. In conclusion, perinatally secreted maternal PRL affects the expression of nurturing behaviors not only in a mother, but also in her pups when they have reached adulthood.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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