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  • 1
    Electronic Resource
    Electronic Resource
    ELECTROFOCUSING OF GRAIN PROTEINS FROM WHEAT GENOTYPES (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 209 (1973), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1973.tb47526.x
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  • 2
    Electronic Resource
    Electronic Resource
    Variation of B subunits of glutenin in durum, wild and less-widely cultivated tetraploid wheats (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three major types of B subunits of glutenin patterns were detected among 240 durum wheat lines collected from eleven countries by the one-step one-dimensional SDS-PAGE procedure. Most commercial durum lines had the LMW-2 type while extensive variation of other banding patterns was found for lines particularly from North African and Mediterranean region. A total of 281 wild and less-cultivated tetraploid wheat lines (var. dicoczoides, Triticum dicoccum and T. polonicum) were also studied for the variation of low molecular weight glutenin subunits and extensive variation was found. The complexity of banding patterns observed among the T. turgidum species indicate a rich source of glutenin variation with a potential value to be used for quality improvement of cultivated wheat when their functional properties have been tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00896.x
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  • 3
    Electronic Resource
    Electronic Resource
    Grain quality and yield characteristics of D-genome disomic substitution lines in ‘Langdon’ (Triticum turgidum var. durum) (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sets of D-genome disomic substitution lines of ‘Langdon’ (Triticum turgidum var. durum) were used to study the effect of chromosome substitutions on grain yield and flour technological properties.In general, the substitution of any D-genome chromosome had a detrimental effect on grain yield and growth vigour (some lines were sterile). SDS-sedimentation, SE-HPLC and two-gram mixograph procedures were used to measure dough strength of the lines studied. Significant correlations were observed between protein concentration and grain yield and other quality parameters such as SDS-sedimentation value, the proportion of glutenin, dough mix time and peak resistance. Most of the quality characters were highly correlated with each other.Substitution of chromosomes 1D, 5D, 2D and 7D resulted in positive responses to SDS-sedimentation values, but only chromosome 1D had positive effects on the proportion of peak 1 (P1%), measured by SE-HPLC. Besides the major influence of chromosome 1D on three major mixograph parameters (mixing time, peak resistance and resistance breakdown), chromosome 5D also exhibited significant effects on these mixing parameters. Principal-component analysis showed that the predominant effect on durum-wheat rheological properties was from chromosome 1D, whereas chromosome 5D had a major effect on grain hardness (50%) and increased the whiteness of the flour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00755.x
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of Rye Chromosome 1R Translocations and Substitutions in Hexaploid Wheats Using Storage Proteins as Genetic Markers (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 109 (1992), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Grain protein compositions of 106 advanced generation backcross lines from crosses involving ‘Amigo’ (1AL.1RS), ‘Aurora’, ‘Kavkaz’, ‘Skorospelka-35’ and ‘Sunbird’ (all 1BL.1RS) and ‘Gabo’ 1DL.1RS parents and 152 cultivars with unknown pedigree were analysed by one-dimensional SDS-PAGE. Eighty seven backcross lines and 16 cultivars carried one or other of these translocations, 2 cultivars had a 1R (1B) substitution, whereas 5 backcross lines were found to be heterogeneous for the 1BL.1RS translocation. The translocation lines were easily identified by the presence of secalins (Sec-1) controlled by rye chromosome arm IRS and a simultaneous loss of the gliadin (Gli-1) and/or triticin (Tri-1) protein bands controlled by the replaced wheat chromosome arm (1AS, 1BS or 1DS). Certain gliadins, showing no allelic variation among the genotypes analysed, were identified as markers for chromosome arms 1AS (Mr= 34 kd) and IBS (Mr= 42,33 kd). The whole chromosome substitutions 1R (1B) were recognized by scoring for the presence of Sec-1 and HMW secalin bands, Sec-3 (controlled by rye chromosome arm 1RL) and the absence of Gli-B1 and HMW glutenin subunits, Glu-B1 (controlled by wheat chromosome arm 1BL). The results have shown that protein electrophoresis provides a rapid and reliable technique for screening genotypes for these translocations and substitutions in a breeding programme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1992.tb00163.x
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  • 5
    Electronic Resource
    Electronic Resource
    Substituting Ability of Individual Barley Chromosomes for Wheat Chromosomes 1. Substitutions Involving Barley Chromosomes 1, 3 and 6 (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 109 (1992), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to determine the genetic relatedness of individual barley chromosomes to wheat chromosomes, ‘Betzes’ barley chromosomes 1, 3 and 6 were substituted for individual ‘Chinese Spring’ wheat chromosomes of homoeologous groups 7, 3 and 6, respectively. The substitution status of these lines has been confirmed using isozyme selective markers, chromosome pairing behaviour in F1 hybrids between the substitution lines and the appropriate double ditelocentric stocks of wheat, and hybridization of cDNA probes to the genomic DNA digests of these substitution lines. Each of the three barley chromosomes provided genetic compensation for the wheat chromosomes they replaced in the substitution plants. From the basis of this compensation with respect to plant vigour and fertility, barley chromosomes 1, 3 and 6 have been designated 7H, 3H and 6H.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1992.tb00164.x
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  • 6
    Electronic Resource
    Electronic Resource
    Inheritance of tolerance to zinc deficiency in barley (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Barley (Hordeum vulgare L.) is often grown on alkaline zinc (Zn)-deficient soils where reductions in yield and grain quality are frequently reported. Currently, the use of Zn-based fertilizer along with Zn-deficiency-tolerant genotypes is considered the most thorough approach for cropping the Zn-deficient soils; however, developing or breeding genotypes with higher Zn efficiency requires a good understanding of the inheritance of tolerance to Zn deficiency. This study was conducted to determine genetic control of this trait in barley. Two parental cultivars ('Skiff, moderately tolerant; and ‘Forrest’, sensitive), 185 F2 plants, and 48 F2-derived F3 families from this cross were screened to determine inheritance of tolerance to Zn deficiency using a visual score of deficiency symptoms. The segregation ratios observed indicated that greater tolerance to Zn deficiency in ‘Skiff compared with ‘Forrest’ at the seedling stage is controlled by a single gene with no dominance. The results also indicate that visual scores are useful for genetic analysis of tolerance to Zn deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00845.x
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  • 7
    Electronic Resource
    Electronic Resource
    Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 609-616 
    Details
    ISSN: 1432-2242
    Keywords: Rye ; Secalins ; Rust resistance ; Stem rust ; Leaf rust ; Stripe/yellow rust ; Linkage mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes controlling resistance to three wheat rusts, viz., leaf rust (Lr26), stem rust (Sr31) and stripe or yellow rust (Yr9), and ω-secalins (Sec1), located on the short arm of rye chromosome 1R, were mapped with respect to each other and the centromere. Analysis of 214 seeds (or families derived from them) from testcrosses between a 1BL.1RS/1R heterozygote and ‘Chinese Spring’ ditelocentric 1BL showed no recombination between the genes for resistance to the three rusts, suggesting very tight linkage or perhaps a single complex locus conferring resistance to the three rusts. The rust resistance genes were located 5.4 ± 1.7 cM from the Sec1 locus, which in turn was located 26.1 ± 4.3 cM from the centromere; the gene order being centromere — Sec1 — Lr26/Sr31/Yr9 — telomere. In a second test-cross, using a different 1BL.1RS translocation which had only stem rust resistance (SrR), the above gene order was confirmed despite a very large proportion of aneuploids (45.8%) among the progeny. Furthermore, a map distance of 16.0 ± 4.8 cM was estimated for SrR and the telomeric heterochromatin (C-band) on 1RS. These results suggest that a very small segment of 1RS chromatin is required to maintain resistance to all three wheat rusts. It should be possible but difficult to separate the rust resistance genes from the secalin gene(s), which are thought to contribute to dough stickiness of wheat-rye translocation lines carrying 1RS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224219
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 537-544 
    Details
    ISSN: 1432-2242
    Keywords: Wheat-rye recombinants ; Homoeologous recombination ; Repetitive DNA sequences ; RFLP markers ; mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The introgression of genetic material from alien species is assuming increased importance in wheat breeding programs. One example is the translocation of the short arm of rye chromosome 1 (1RS) onto homoeologous wheat chromosomes, which confers disease resistance and increased yield on wheat. However, this translocation is also associated with dough quality defects. To break the linkage between the desirable agronomic traits and poor dough quality, recombination has been induced between 1RS and the homoeologous wheat arm IDS. Seven new recombinants were isolated, with five being similar to those reported earlier and two havina new type of structure. All available recombinantsw ere characterized with DNA probes for the loci Nor-R1, 5SDna-R1, and Tel-R1. Also, the amount of rye chromatin present was quantified with a dispersed rye-specific repetitive DNA sequence in quantitative dot blots. Furthermore, the wheat-rye recombinants were used as a mapping tool to assign two RFLP markers to specific regions on chromosome arms 1DS and 1RS of wheat and rye, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226788
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  • 9
    Electronic Resource
    Electronic Resource
    Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheats using these stocks (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 719-728 
    Details
    ISSN: 1432-2242
    Keywords: Rye translocations ; Triticum aestivum ; Glutenin ; Gliadin ; Glu-3 loci ; Gli-1 loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A triple (1AL.1RS/1BL.1RS/1DL.1RS) and three double (1AL.1RS/1BL.1RS, 1AL.1RS/1DL.1RS, 1BL.1RS/1DL.1RS) wheat-rye 1RS translocation stocks were isolated from a segregating population using the Gli-1, Tri-1 and Sec-1 seed proteins as genetic markers. These stocks carried 42 chromosomes and formed the expected multivalents (frequency of 14–25%) at metaphase 1. They gave floret fertility ranging from 40–60%. These stocks were subsequently used to determine the genetic control of low-molecular-weight (LMW) glutenin subunits in ‘Chinese Spring’ and ‘Gabo’ by means of two-step one-dimensional SDS-PAGE. All of the B subunits and most of the C subunits of glutenin were shown to be controlled by genes on the short arms of group-1 chromosomes in these wheats. The other C subunits were not controlled by group-1 chromosomes. The triple translocation line served as a suitable third parent in producing test-cross seeds for studying the inheritance of the LMW glutenin subunits and gliadins in wheat cultivars, e.g. ‘Chinese Spring’ and ‘Orca’. The segregation patterns of the LMW glutenin subunits in these cultivars revealed that the subunits were inherited in clusters and that their controlling genes (Glu-3) were tightly linked with those controlling gliadins (Gli-1). The LMW glutenin patterns d, d and e in ‘Orca’ segregated as alternatives to the patterns a, a and a in ‘Chinese Spring’ controlled by Glu-A3, Glu-B3 and Glu-D3 loci on chromosome arms 1AS, 1BS and 1DS, respectively, thus indicating that these patterns were controlled by allelic genes at these loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225011
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  • 10
    Electronic Resource
    Electronic Resource
    Meiotic restitution in wheat-barley hybrids (1980)
    Springer
    Chromosoma 79 (1980), S. 363-372 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Meiotic restitution occurs in pollen mother cells (PMCs) of reciprocal F1 hybrids between wheat and barley. In occasional PMCs, all or most of the 28 chromosomes assemble at the equatorial plate at metaphase I, but instead of undergoing anaphase I separation they reform into a mass of chromatin to form a restitution nucleus. Some of these restituted nuclei undergo a regular second division and dyads are produced among other non-restituted cells which have reached the tetrad stage of division. Other restituted nuclei fail to undergo a second division and then the PMCs appear as monads among neighbouring tetrads. Both the monads and dyads are expected to produce microsporocytes with the diploid complement of chromosomes. Chromosomes which fail to become included in the restituted nucleus form separate micronuclei and, depending on whether they undergo a regular second division or not, the PMCs containing them eventually appear as tetrads, triads or dyads. These partially restituted nuclei are expected to produce unreduced gametes, deficient for one or more chromosomes. It is postulated from these observations that restitution in wheatbarley F1 hybrids depends on a high frequency of univalent accumulation at the equatorial plate at metaphase I and the subsequent failure of the chromosomes to undergo anaphase I separation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327326
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