ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Calcium concentration and intracellular localization were investigated using the electron microscopic X-ray analyser (EMMA-4) in preparations of dog myocardial tissues fixed either in glutaraldehyde, osmium tetroxide or potassium pyroantimonate plus osmium tetroxide. Calcium could be detected with all the fixatives, but at varying efficiency. Glutaraldehyde fixation showed small detectable amounts of calcium and without special localization except in the nucleus. Osmium fixation showed much higher, but still poorly localized concentrations. The pyroantimonate technique produced the highest calcium concentrations and loci of statistically significant concentrations. Preparations stained with uranyl acetate or lead citrate showed well-visualized precipitates but no detectable calcium concentrations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00499662
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