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  • 1
    Electronic Resource
    Electronic Resource
    Helper function of T7 protein kinase in virus propagation (1975)
    [s.l.] : Nature Publishing Group
    Nature 255 (1975), S. 508-510 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] From the above considerations, one would have expected that the absence of protein kinase would have serious effects on phage development. The contrary was found, however, and the burst size or latent period in infection did not depend on the presence of protein kinase1'2. Furthermore, one widely ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/255508a0
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  • 2
    Electronic Resource
    Electronic Resource
    Deficiency of DNA ligase activity in Fanconi's anemia (1978)
    Springer
    Human genetics 〈Berlin〉 45 (1978), S. 25-32 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A significant decrease in DNA ligase activity was observed in lymphocytes and fibroblasts of a patient with Fanconi's anemia (FA). This decrease is related to the observed DNA repair deficiency indicated by the delayed closing of repair DNA strands following UV irradiation. Other steps of DNA repair were analyzed in the FA fibroblasts, including endonucleolytic incision of DNA, repair DNA synthesis, and exonucleolytic removal of the photoproducts. No differences were found against control cells. The action of DNA ligase is delayed during replication in the FA cells, as seen by an accumulation of replicative intermediates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277570
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  • 3
    Electronic Resource
    Electronic Resource
    Intracellular distribution of DNA topoisomerase I in fibroblasts from patients with Fanconi's anaemia (1982)
    Springer
    Human genetics 〈Berlin〉 61 (1982), S. 369-371 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The activity of DNA topoisomerase I (DNA nicking-closing enzyme) was analysed in cytoplasmic and nuclear extracts of six independently derived Fanconi and four normal fibroblast cell lines. In all experiments the total cellular activity was predominantly found in the nuclear extracts (88–100%). In addition, a minor proportion of the enzyme (up to 12%) was randomly present in some of the cytoplasmic fractions of both Fanconi and normal fibroblasts. These results indicate that Fanconi's anaemia is probably not due to or accompanied by a maldistribution of topoisomerase I between nuclei and cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276603
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  • 4
    Electronic Resource
    Electronic Resource
    Clinical and biochemical studies in three patients with severe early infantile Cockayne syndrome (1989)
    Springer
    Human genetics 〈Berlin〉 83 (1989), S. 339-346 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We present clinical and biochemical data from three patients with severe Cockayne syndrome (CS) of very early onset. Unlike in classic CS, signs became evident in the first weeks of life and led to unusually early death. Fibroblasts from two of the patients showed a complete defect of the repair of UV-induced thymine dimer lesions. They were unable to remove thymine dimer lesions from their DNA, had a severe reduction of the RNA synthesis rates after UV irradiation, and showed no reactivation of an UV-inactivated indicator gene and no DNA recondensation after UV irradiation. DNA repair investigated in these two fibroblast cell strains resembled that of xeroderma pigmentosum cells of complementation group A. In contrast, fibroblasts from the third patient showed the same in vitro repair characteristics as classic CS cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291378
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  • 5
    Electronic Resource
    Electronic Resource
    No abnormalities in the NAD+ ADP-ribosyltransferase (polymerizing) gene of transformed cells from a Fanconi's anemia patient (1992)
    Springer
    Human genetics 〈Berlin〉 89 (1992), S. 690-691 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221967
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  • 6
    Electronic Resource
    Electronic Resource
    The role of oxygen metabolism for the pathological phenotype of Fanconi anemia (1997)
    Springer
    Human genetics 〈Berlin〉 99 (1997), S. 710-719 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The molecular defect of the hereditary disease Fanconi anemia (FA) remains unknown. The two theoretical possibilities are (1) an impaired DNA crosslink-repair system or (2) a disturbed oxygen metabolism either by overproduction of reactive oxygen intermediates (ROI) or by diminished detoxification of ROI. In order to gain further insight into the molecular mechanism of this disease, we have determined the repair capacity of FA cells challenged by crosslinking agents and have analyzed diverse biological systems that are involved in oxygen metabolism. We have tested normal and FA cells for oxygen consumption and for the activity of the antioxidant phospholipid-hydroperoxide-glutathione-peroxidase (PHGPx). FA cells show a reduced oxygen consumption and an increased PHGPx activity. Since spontaneous and induced chromosomal instability is a main cellular feature of FA, we have analyzed the redox state of cells and the effect of cytochrome P-450 (Cyt P-450) inhibitors and inducers on chromosomal breaks and micronuclei production. Our results indicate that Cyt P-450 enzymes, especially Cyt P-450 1A2, play a crucial role in radical metabolism in FA cells. Furthermore, we have determined NF-κB activity in untransformed cells and in SV40-transformed cells by gel shift experiments. NF-κB is a multiunit transcription factor that is known to be induced by ROI and that activates the expression of various genes involved in cellular responses to stress. NF-κB is constitutively induced in SV40-transformed FA cells probably as a consequence of an increased ROI level. Our results suggest that enzymatic defects in oxygen metabolism mediate the FA phenotype via impaired reactivity with ROI. Cyt P-450 1A2 appears to be a good candidate for the defective enzyme, even though no differences have been measured in the activity of this enzyme in FA and control fibroblasts in pilot experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050437
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  • 7
    Electronic Resource
    Electronic Resource
    Difference in the location inDasycladaceae of a DNA sequence homologous to theDrosophila per locus (1989)
    Springer
    Plant cell reports 8 (1989), S. 169-173 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The period (per) locus ofDrosophila melanogaster has a fundamental role on the expression of biological rhythms. A DNA sequence, which is homologous to a short region of theDrosophila per locus, has been found at different locations in various species of Dasycladaceae. InBatophora oerstedii, one of the phylogenetically oldest member of Dasycladaceae, a DNA sequence homologous to theDrosophila per locus was detected only in the chloroplast genome but not in the nuclear genome. In contrast, inAcetabularia cliftonii which in phylogeny branched off Batophora 350 million years ago, like in higher plants, theper locus homologous sequence is located in the nuclear rather than the chloroplast genome. The difference in the location of this sequence in phylogenetically separated species of the ancient unicellular and uninucleate green algae suggests gene translocation between the chloroplast genome and the nuclear genome during evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716833
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  • 8
    Electronic Resource
    Electronic Resource
    Transcriptional regulation and autoregulation of the human gene for ADP-ribosyltransferase (1994)
    Springer
    Molecular and cellular biochemistry 138 (1994), S. 99-104 
    Details
    ISSN: 1573-4919
    Keywords: poly(ADP-ribosyl) transferase (human) ; autoregulation ; gene expression ; promoter structure ; cruciform structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Human nuclear poly(ADP-ribosyl)transferase (ADPRT) modifies proteins with branched ADP-ribose-polymers. Various proteins, including ADPRT itself, serve as acceptors for polyADP-ribose. Target proteins include those controlling basic cellular processes such as DNA repair, differentiation and proliferation. Because of the outstanding features of this enzyme: automodification, several functional domains and central role in physiology of the cell, the molecular biology of ADPRT gained wide interest. The promoter structure contains several CCAAT/TATA boxes and SP1 sites. However, there is no CCAAT/TATA box in the neighbourhood of an SP1 site and, thus no obvious site for initiation of transcription. Within this region there are several noteworthy inverted repeats, which by internal basepairing could form two types of cruciform structures. Deletion analysis revealed that these cruciform structures have functional significance. Removal of one type increases the promoter activity, whereas removal of the other diminishes the promoter function. Overexpression of ADPRT from heterologous promoters (MMTV, SV40) leads to repression of the activity of the ADPRT promoter. Indeed, ADPRT was shown to bind specifically to one type of cruciform structure. This specific interaction indicates autorepression of the ADPRT gene: the enzyme ADPRT acts directly as a negative modulator of the activity of its own promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928449
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  • 9
    Electronic Resource
    Electronic Resource
    On the molecular mechanism of the circadian clock: The 64000-Mr protein of Chlamydomonas reinhardtii might be related to the biological clock (1992)
    Springer
    Planta 186 (1992), S. 593-599 
    Details
    ISSN: 1432-2048
    Keywords: Chlamydomonas (circadian rhythm) ; Circadian rhythm ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Labelling of Chlamydomonas reinhardtii cells with [35S] methionine led to the detection of a 64-kDa polypeptide which is synthesized according to a circadian rhythm. The change in synthesis rate could be demonstrated to exist under constant dim-light conditions as well as in darkness. Maximum synthesis of the 64-kDa polypeptide occurred at about 10 h after onset of constant conditions, and the period length of its oscillation was about 29 h. The 64-kDa polypeptide was synthesized on 80S ribosomes as shown by experiments in which cycloheximide and chloramphenicol were supplied to the cultures. Peptide-microsequence analysis yielded an N-terminal sequence of 14 amino acids. No significant homology to any other known polypeptide could be demonstrated in searches of current databases. The possible role of the 64-kDa polypeptide and its relationship to the biological clock is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198041
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  • 10
    Electronic Resource
    Electronic Resource
    RNA polymerase synthesis in vitro directed by T7 phage DNA (1971)
    Springer
    Molecular genetics and genomics 110 (1971), S. 31-35 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary T7 RNA polymerase is synthesized in vitro, dependent on T7 DNA. The in vitro synthesized T7 polymerase has the characteristic properties: resistance to rifampicin and streptolydigin and the typical template specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276043
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