Publication Date:
1985-07-12
Description:
The mechanism of site-specific genetic recombination mediated by Tn3 resolvase has been investigated by a topological approach. Extrapolation of a detailed model of synapsis and strand exchange predicts the formation of an additional DNA product with a specific knotted structure. Two-dimensional gel electrophoresis of DNA reacted in vitro revealed a product, about 0.1 percent of the total, with the appropriate mobility. A technique for determining DNA topology by electron microscopy was improved such that less than a nanogram of DNA was required. The structure of the knot was as predicted, providing strong evidence for the model and showing the power of the topological method.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wasserman, S A -- Dungan, J M -- Cozzarelli, N R -- GM 07232/GM/NIGMS NIH HHS/ -- GM 31655/GM/NIGMS NIH HHS/ -- GM 31657/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Jul 12;229(4709):171-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2990045" target="_blank"〉PubMed〈/a〉
Keywords:
Electrophoresis
;
Microscopy, Electron
;
*Models, Genetic
;
Models, Molecular
;
Nucleotidyltransferases/*metabolism
;
*Recombination, Genetic
;
Transposases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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