ISSN:
1476-4687
Source:
Nature Archives 1869 - 2009
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Notes:
[Auszug] We have purified tubulin from porcine brain by a modification of the methods used by Eipper10 and by Luduena et al.1. The 100,000g brain supernatant in 0.05 M sodium pyrophosphate buffer, pH 7.0, was incubated with 0.1 mM colchicine for 15 min at 37 C before chromatography on DEAE-cellulose with a ...
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/282423a0
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