ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary An ultrastructural modification and application of the sulfide-silver method for cell suspensions and ultrathin sections of tissue has been described using glutaraldehyde saturated with hydrogen sulfide as a fixative. When, after physical development in a modified sulfide-silver developer, silver grains appeared in fully treated cells and sections but neither in ungassed but developed controls, nor in gassed but non-developed sections, they were thought to give the sites of heavy metals. A positive reaction for heavy metals has been described to occur in the secretion granules of pancreatic β-cells of dog, cat, Chinese hamster, rabbit, and rat, as well as the fish Cottus quadricornis. On isolated cells a positive reaction was obtained in the fungus Cryptococcus neoformans, on the surface of all rat peritoneal cells and cattle blood leucocytes, as well as in erythrocytes and in the specific granules of rat mast cells and rat and cattle eosinophils. The limitations and critical methodological points of the procedure are given, as well as the precautions needed in the interpretation of the results.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00311403
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