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  • 1
    Electronic Resource
    Electronic Resource
    The HMW Glutenin Subunit and Gliadin Compositions of German-Grown Wheat Varieties and their Relationship with Bread-Making Quality (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 103 (1989), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The HMW glutenin subunit and gliadin compositions of 57 German-grown varieties of wheat were determined by 1D- and 2D-polyacrylamide gel electrophoresis, respectively. A Glu-1 quality score was calculated for each variety based on previously observed associations between individual HMW glutenin subuints and SDS-sedimentation volume. The results indicated that approximately 30% of the variation in bread-making quality of the varieties was accounted for by variation in the Glu-1 quality score, and that this proportion was raised to 54 % if the presence or absence of chromosome 1BL.1RS was taken into account. It was also shown that allelic variation at the Gli-1 and Gli-2 loci had a relatively minor effect on quality; indeed, the only significant effect was associated with variation at Glu-1, and the influence of this locus was principally due to the deterimental effect of 1BL.1RS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1989.tb00356.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetics of Wheat Storage Proteins and the Effect of Allelic Variation on Bread-Making Quality (1987)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 38 (1987), S. 141-153 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.38.060187.001041
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  • 3
    Electronic Resource
    Electronic Resource
    Free and membrane bound ribosomes of the cotyledons of Vicia faba (L.) (1969)
    Springer
    Planta 84 (1969), S. 263-271 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes in the quantities of free and membrane-bound ribosomes were followed in the cotyledons of developing seeds. During the phase of storage protein synthesis, free and membrane bound ribosomes do not interchange, and as both classes synthesise protein in vivo, it is possible that they may synthesise different groups of proteins. This suggestion is discussed in relationship to the developing cotyledon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388112
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  • 4
    Electronic Resource
    Electronic Resource
    Katabolism of plant cytoplasmic ribosomes: A study of the interaction between ribosomes and ribonuclease (1974)
    Springer
    Planta 117 (1974), S. 259-268 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ribosome preparations from several species of flowering plant were found to contain ribonuclease. It was predominantly latent in ionic conditions which maintained the integrity of the ribosomes but was activated by agents which disrupted ribosome structure by chelating their divalent cations. The ribonuclease was not a structural component of the ribosomes but was preferentially adsorbed by them upon disruption of cells. Adsorption took place onto both large and small ribosomal subunits, and onto monosomes and polysomes. These results are discussed in relation to possible mechanisms for the breakdown of ribosomal RNA in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388399
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  • 5
    Electronic Resource
    Electronic Resource
    Katabolism of plant cytoplasmic ribosomes: RNA breakdown in senescent cotyledons of germinating broad-bean seedlings (1974)
    Springer
    Planta 117 (1974), S. 251-258 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Broad-bean cotyledons lost about 80% of their RNA during the first 30 days of seed germination. Both high-molecular-weight ribosomal (r) RNA and low-molecular-weight RNA were degraded at about the same rate. Only small quantities of breakdown products of rRNA were detected within senescent tissue. This finding contrasts strongly with the very rapid cleavage of rRNA and the accumulation of breakdown products when the same cotyledons were homogenised and then incubated at 25°. It would appear, therefore, that there was a barrier between ribonuclease and most ribosomes in vivo which was disrupted by homogenisation. The results are discussed in relation to the mechanism of ribosome katabolism during senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388398
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of the ribosomal ribonucleic acids of blue-green algae (1972)
    Springer
    Archives of microbiology 87 (1972), S. 29-40 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal RNA components of 12 species of blue-green algae have been characterized. The 23S RNA of most species is labile and discrete cleavage products were detected by polyacrylamide gel electrophoresis. In contrast, the 23S and 16S RNA's of three species, Anacystis nidulans, Nostoc sp. and Oscillatoria tenuis were essentially undegraded (apart from a “hidden” break in some of the 23S RNA molecules) and these are the most suitable species for further study. The undegraded 23S and 16S RNA's have similar molecular weights (1.07×106 and 0.53–0.54×106 respectively) to the corresponding molecules from bacteria and eukaryote chloroplasts. The nucleotide base compositions of separated, intact, 23S and 16S RNA's from blue-green algae are also of the prokaryotic type. For instance, the (G+C) content of each RNA is approximately 52 moles % and the (G-C)+(A-U) values are high (16–24 moles %). Blue-green algae, like other organisms, contain a 5S ribosomal RNA. Its electrophoretic mobility in polyacrylamide gels and its behaviour on methylated-albumen-kieselguhr-columns relative to E. coli, plant cytoplasmic and plant chloroplast 5S RNA's, are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424776
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  • 7
    Electronic Resource
    Electronic Resource
    Chromosomal location of genes coding for endosperm proteins of Hordeum chilense, determined by two-dimensional electrophoresis of wheat-H. chilense chromosome addition lines (1987)
    Springer
    Biochemical genetics 25 (1987), S. 53-65 
    Details
    ISSN: 1573-4927
    Keywords: Hordeum chilense ; endosperm protein genes ; two-dimensional electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The proteins of Hordeum chilense grain were resolved into 25 major components by two-dimensional electrophoresis. Their solubilities in aqueous alcohol solutions were determined to distinguish prolamin storage proteins from metabolic and structural proteins. The prolamins were divided into two groups, based on the presence or absence of intermolecular disulfide bonds determined by gel-filtration chromatography. Using an incomplete set of Chinese Spring wheat-H. chilense disomic addition lines, the structural genes of 21 of the 26 most dominant seed proteins were assigned to chromosomes. The great majority of the prolamin genes, including those coding for a high molecular weight (HMW) prolamin subunit, was present on chromosome 1Hch. However, a small number of prolamin genes also occurred on chromosomes 5Hch and 7Hch. A minor protein, probably belonging to the nonstorage group of proteins, is coded by genes on 5Hch. Various ditelosomic addition lines and ditelosomic and disomic substitution lines for chromosome 7Hch were also analyzed by electrophoresis. This technique revealed that the genes for three major prolamins occur on the β arm of chromosome 7Hch and that a gene for a minor protein, also thought to be a prolamin, occurs on the α arm. These results are discussed in relation to the evolution of prolamin genes in the Triticeae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00498951
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  • 8
    Electronic Resource
    Electronic Resource
    Free and membrane-bound ribosomes of the cotyledons of Vicia faba (L.) (1969)
    Springer
    Planta 87 (1969), S. 63-68 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary During the first few days of germination, the RNA content of the cotyledons remained approximately constant, but the quantity of membrane-bound ribosomes increased. Experiments with orthophosphate-32P indicated that these ribosomes were synthesised de novo, and did not originate by the attachment to membranes of pre-existing free ribosomes. This conclusion was discussed in relation to the suggestion that free and membrane-bound ribosomes synthesise different groups of proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386965
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  • 9
    Electronic Resource
    Electronic Resource
    The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum) (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 103-111 
    Details
    ISSN: 1432-2242
    Keywords: Pollen irradiation ; Wheat ; Differential gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The use of irradiated pollen to bring about limited gene transfer in wheat has been investigated. Doses of X-rays of 2Kr, 3Kr and 5Kr were used to generate M1 progeny between maternal and paternal genotypes differing in quantitative and major gene characters. Cytological studies of M1 plants revealed hybrids with widespread aneuploidy and structural rearrangements in the paternal genome. These effects resulted in phenotypic variation between M1 progeny and complex multivalent formation at meiosis. All M1 plants at the 5Kr and 3Kr doses were sterile and all but 2 plants at the 2Kr dose. Studies of the two M2 families from these plants revealed disturbances in genotype frequencies for some of the marker loci with an excess of maternal homozygotes and a deficit of paternal homozygotes. This was also reflected in a more maternal appearance for quantitative characters. These results are interpreted as showing that irradiation damage to the paternal genome in M1 plants results in the differential transmission of maternal alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264875
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  • 10
    Electronic Resource
    Electronic Resource
    Development and characterisation of a line of bread wheat, Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 327-334 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Triticum ; Polyacrylamide gel-electrophoresis ; Light and electron microscopy ; Chromosome deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary About 360 offspring of a tri-parental cross were screened by gel electrophoresis and unexpectedly one of them did not contain chromosome 1B ω-gliadins derived from either of the primary parents. A line disomic for the ω-gliadin null was developed from the surviving embryo half of the unique grain. Two dimensional electrophoresis revealed that all the storage protein genes at Gli-B1, coding for γ-gliadins, β-gliadins and low-molecular-weight subunits of glutenin as well as the ω-gliadin, were not expressed. The nuclei of dividing root-tip cells were shown by light microscopy to lack the normal short-arm satellites of chromosome 1B, indicating that the genes for the missing storage proteins had been lost through a terminal deletion. Using a radioactive ribosomal RNA probe, the deficient 1B chromosomes were shown to contain ribosomal RNA genes demonstrating that at least two-thirds of the short arm was still present. Examination of serial sections of chromosome 1B at metaphase by low-power electron microscopy showed that the point of scission of this chromosome was within the secondary constriction where the ribosomal RNA genes are located. The Gli-B1 locus must therefore be carried on the short-arm satellite. Transmission of the deficient chromosome from female gametes to progeny was normal (i.e., about 50%) but from pollen it was poor (8.8%). Recombination mapping indicated that the distance from the ribosomal RNA genes (Nor1) to Glu-B1 was 22 cM, equivalent to 13 cM from Nor1 to the centromere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267886
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