ISSN:
1573-4986
Keywords:
Tamm-Horsfall protein
;
carbohydrate-deficient glycoprotein syndrome
;
glycans
;
glycoproteins
;
CBB, Coomassie Brilliant Blue R-250
;
CDG, carbohydrate-deficient glycoprotein syndrome
;
DIG, digoxigenin
;
ELISA, enzyme-linked immunosorbent assay
;
GU, glucose units
;
PBS, phosphate buffered saline, pH 7.4
;
PMM, phosphomannomutase
;
SDS, sodium dodecyl sulfate
;
SDS-PAGE, SDS polyacrylamide gel electrophoresis
;
THP, Tamm-Horsfall protein
;
TPBS, 0.1% Tween 20 in PBS
;
MAA, Maackia amurensis lectin
;
SNA, Sambucus nigra lectin
;
DSA, Datura stramonium lectin
;
AAA, Aleuria aurantia lectin
;
ConA, Canavalia ensiformis lectin
;
PHA-L, Phaseolus vulgaris lectin
;
GNA, Galanthus nivalis lectin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract As the sugar moiety of Tamm-Horsfall protein (THP) is affected by many pathological conditions, the aim of this study was to examine the influence of carbohydrate-deficient glycoprotein syndrome (CDG) on THP glycans. THP was isolated from urine of one patient with CDG type I and N-glycan profiling, analysis of monosaccharide content, determination of THP reactivity with specific lectins and with anti-THP antibodies were performed. THP of the CDG patient showed markedly lower amounts of all monosaccharides. Diminished amounts of lactosamine-type chains, galactose and α2,3 linked sialic acid were expressed in lower reactivity with PHA-L, DSA and MAA, respectively. These modifications were reflected in altered proportions of tetrasialylated and disialylated oligosaccharide chains. THP of the CDG patient reacted slightly more with anti-THP antibodies. Our results indicate that the CDG type I affects the THP sugar moiety and slightly enhances the THP immunoreactivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007039619484
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