ISSN:
0935-6304
Keywords:
Gas liquid chromatography, GLC
;
Capillary columns
;
Optical emission spectroscopy
;
Microwave-induced plasma detector, MIPD
;
Organofluorine compounds
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Fluorinated analogs of compounds typical of those found in metabolic and other biological studies are detected with high selectivity using a gas chromatograph/microwave-induced plasma detector (GC-MIPD), which permits fluorine-selective detection by monitoring the emission at 685.6 nm. Using the described atmospheric pressure helium-sustained plasma detector, the minimum detectable level, fluorine selectivity (relative to carbon), and linear dynamic range of this GC-MIPD system were determined to be 4.8 pg-F/s, 1060, and 5000, respectively. The utility of this GC-MIPD system for the selective detection of organofluorine compounds is demonstrated by its application in the analysis of the metabolic fate of a fluorinated substrate administered to a mixture of wheat germ phosphatase and potato apyrase, as well as by analysis of synthetic mixtures.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240110502
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