Publication Date:
2007-04-07
Description:
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837481/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837481/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goshima, Gohta -- Wollman, Roy -- Goodwin, Sarah S -- Zhang, Nan -- Scholey, Jonathan M -- Vale, Ronald D -- Stuurman, Nico -- R37 GM038499/GM/NIGMS NIH HHS/ -- R37 GM038499-21/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2007 Apr 20;316(5823):417-21. Epub 2007 Apr 5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17412918" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
Centrosome/metabolism/ultrastructure
;
Chromosomes/physiology/ultrastructure
;
Drosophila Proteins/*genetics/*physiology
;
Drosophila melanogaster
;
*Genes, Insect
;
Image Processing, Computer-Assisted
;
Kinetochores/metabolism
;
Metaphase
;
Microtubules/metabolism
;
Mitosis
;
Phenotype
;
RNA Interference
;
Spindle Apparatus/*genetics/*metabolism/ultrastructure
;
Tubulin/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink