Publication Date:
2012-04-21
Description:
Prion conversion from a soluble protein to an aggregated state may be involved in the cellular adaptation of yeast to the environment. However, it remains unclear whether and how cells actively use prion conversion to acquire a fitness advantage in response to environmental stress. We identified Mod5, a yeast transfer RNA isopentenyltransferase lacking glutamine/asparagine-rich domains, as a yeast prion protein and found that its prion conversion in yeast regulated the sterol biosynthetic pathway for acquired cellular resistance against antifungal agents. Furthermore, selective pressure by antifungal drugs on yeast facilitated the de novo appearance of Mod5 prion states for cell survival. Thus, phenotypic changes caused by active prion conversion under environmental selection may contribute to cellular adaptation in living organisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Suzuki, Genjiro -- Shimazu, Naoyuki -- Tanaka, Motomasa -- New York, N.Y. -- Science. 2012 Apr 20;336(6079):355-9. doi: 10.1126/science.1219491.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory for Protein Conformation Diseases, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22517861" target="_blank"〉PubMed〈/a〉
Keywords:
Alkyl and Aryl Transferases/*chemistry/genetics/*metabolism
;
Antifungal Agents/*pharmacology
;
Biosynthetic Pathways
;
Crosses, Genetic
;
Drug Resistance, Fungal
;
Ergosterol/biosynthesis
;
Fluorouracil/pharmacology
;
Microbial Viability
;
Prions/*chemistry/genetics/metabolism
;
Protein Conformation
;
Protein Structure, Tertiary
;
RNA, Fungal/metabolism
;
RNA, Transfer/metabolism
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Saccharomyces cerevisiae/chemistry/*drug effects/genetics/*physiology
;
Saccharomyces cerevisiae Proteins/*chemistry/genetics/*metabolism
;
Selection, Genetic
;
Solubility
;
*Stress, Physiological
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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