Publication Date:
2022-05-25
Description:
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Marine Environmental Research 66 (2008): 15-18, doi:10.1016/j.marenvres.2008.02.010.
Description:
We have cloned the full-length cDNA of the first member of a new cytochrome P450
(CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained
based on an initial 331 bp fragment previously identified among the list of the differentially
expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has
an open reading frame of 1500 bp and based on its deduced aminoacid sequence was
classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed
that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semiquantitative
RT-PCR was performed to analyze the CYP356A1 expression in different
tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed
slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues,
indicating a possible role of the CYP356A1 in the xenobiotic biotransformation and/or
steroid metabolism.
Description:
This work was supported by CNPq-Universal to ACDB. ACDB is recipient of Productivity
Fellowship from CNPq.
Keywords:
Cytochrome P450
;
Pacific oyster
;
Crassostrea gigas
;
Biotransformation enzymes
;
CYP356A1
;
CYP17
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
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