ISSN:
0148-7280
Keywords:
oocyte growth
;
oocyte culture
;
vitellogenin
;
insulin
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
A defined medium for growing defolliculated Xenopus laevis oocytes was developed by monitoring vitellogenin incorporation. Optimum conditions were achieved by use of 50% Liebovitz L-15 medium supplemented with 1 mM L-glutamine, 15 mM Hepes, 5 mg/ml vitellogenin, and 1 μg/ml insulin (final pH = 7.8). Stage IV/V oocytes remained morphologically normal in this medium for at least two weeks and grew at an average rate of 0.25 mm3 per week. This is the first time defolliculated Xenopus laevis oocytes have been grown in vitro.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120010307
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