ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract An expression vector, pFC1, has been constructed based on the promiscuous plasmid RSF1010, which provides autonomous replication in several cyanobacteria of the generaSynechocystis andSynechococcus [Mermet-Bouvier et al., Curr Microbiol 26:323–327]. pFC1 harbors the λcI857 repressor-encoding gene andp R promoter, followed by the λcro ribosome-binding site and ATG translation initiation codon. The latter is located within the uniqueNdeI restriction site (CATATG) of pFC1 and can be exposed after cleavage with this enzyme for in-frame fusion with the protein-coding sequence to be expressed. TheEscherichia coli lacZ reporter gene cloned in pFC1 appeared to be highly expressed in heat-inducedE. coli or cyanobacterial cells. In every case, β-galactosidase amounted to at least 10% of soluble proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01571055
Permalink