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  • 1
    ISSN: 1432-072X
    Keywords: Xanthobacter ; Taxonomy ; Methylotrophs ; Methanol ; Calvin cycle ; Carbon dioxide fixation ; RuBisC/O ; Regulation ; Continuous culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract All Xanthobacter strains studied are versatile autotrophic bacteria, able to grow on methanol and other substrates. Strain 25a, a yellow-pigmented, pleomorphic, Gram-negative bacterium, capable of autotrophic growth on methanol, formate, thiosulfate, and molecular hydrogen, was isolated from an enrichment culture inoculated with soil from a subtropical greenhouse. Subsequent studies showed that the organism also grows on a wide range of multicarbon substrates. Ammonia, nitrate and molecular nitrogen were used as nitrogen sources. The taxonomic relationship of strains H4-14 and 25a with previously described Xanthobacter strains was studied by numerical classification. Strain H4-14 was identified as a X. flavus strain, but the precise position of strain 25a remained uncertain. It probably belongs to a new species of the genus Xanthobacter. The levels of various enzymes involved in autotrophic and heterotrophic metabolism were determined following growth of strains H4-14 and 25a in batch and continuous cultures. The mechanisms involved in controlling ribulose-1,5-bisphosphate carboxylase/oxygenase synthesis in Xanthobacter strains appear to be comparable to those observed for other autotrophic bacteria, namely repression by organic compounds and derepression by autotrophic energy sources, such as methanol and hydrogen.
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  • 2
    ISSN: 1432-072X
    Keywords: Xanthobacter ; Methanol ; Formate ; Methylotrophy ; Autotrophy ; Calvin cycle ; Regulation ; Continuous culture ; RuBisC/O ; Carbon dioxide fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regulation of C1-metabolism in Xanthobacter strain 25a was studied during growth of the organism on acetate, formate and methanol in chemostat cultures. No activity of methanol dehydrogenase (MDH), formate dehydrogenase (FDS) or ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisC/O) could be detected in cells grown on acetate alone over a range of dilution rates tested. Addition of methanol or formate to the feed resulted in the immediate induction of MDH and FDH and complete utilization (D=0.10 h-1) of acetate and the C 1-substrates. The activities of these enzymes rapidly dropped at the higher growth rates, which suggests that their synthesis is further controlled via repression by “heterotrophic” substrates such as acetate. Synthesis of RuBisC/O already occurred at low methanol concentrations in the feed, resulting in additive growth yields on acetate/methanol mixtures. The energy generated in the oxidation of formate initially allowed an increased assimilation of acetate (and a decreased dissimilation), resulting in enhanced growth yields on the mixture. RuBisC/O activity could only be detected at the higher formate/acetate ratios in the feed. The data suggest that synthesis of RuBisC/O and CO2 fixation via the Calvin cycle in Xanthobacter strain 25 a is controlled via a (de)repression mechanism, as is the case in other facultatively autotrophic bacteria. Autotrophic CO2 fixation only occurs under conditions with a diminished supply of “heterotrophic” carbon sources and a sufficiently high availability of suitable energy sources. The latter point is further supported by the clearly more pronounced derepressing effect exerted by methanol compared to formate.
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  • 3
    ISSN: 1432-072X
    Keywords: Key words Dimethylsulfoniopropionate ; Dimethylsulfide ; Acrylate ; Anaerobic respiration ; Sulfate-reducing bacterium ; Desulfovibrio acrylicus sp. nov.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From anoxic intertidal sediment, a dimethylsulfoniopropionate (DMSP)-cleaving anaerobe (strain W218) was isolated that reduced the acrylate formed to propionate. The bacterium was vibrio- to rod-shaped and motile by means of multiple polar flagella. It reduced sulfate, thiosulfate, and acrylate, and used lactate, fumarate, succinate, malate, pyruvate, ethanol, propanol, glycerol, glycine, serine, alanine, cysteine, hydrogen, and formate as electron donors. Sulfate and acrylate were reduced simultaneously; growth with sulfate was faster than with acrylate. Extracts of cells grown in the presence of DMSP contained high DMSP lyase activities (9.8 U/mg protein). The DNA mol% G+C was 45.1. On the basis of its characteristics and the 16S rRNA gene sequence, strain W218 was assigned to a new Desulfovibrio species for which the name Desulfovibrio acrylicus is proposed. A variety of other sulfate-reducing bacteria (eight of them originating from a marine or saline environment and five from other environments) did not reduce acrylate.
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  • 4
    ISSN: 1573-739X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 193 (1994), S. 187-212 
    ISSN: 1615-6110
    Keywords: Rafflesiaceae ; Ovule ; seed structure ; seed dispersal ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genera of theRafflesiaceae show a marked diversity in the structure of their ovules and seeds. Evolutionary trends are recognizable in ovule orientation and number of integuments. A change from anatropous ovules inApodantheae andMitrastemoideae towards incomplete anatropy inRafflesieae and orthotropy inCytineae occurs, next to a change from bitegmic ovules inApodantheae towards unitegmy with rudimentary outer integuments inRafflesieae andCytineae and full unitegmy inMitrastemoideae.—The differences in ovule structure are clearly reflected in the seeds. The seeds are essentially exotegmic, have very small embryos and an oily endosperm.—Seed structure strongly confirms the existing subfamilial classification and supports additional arguments for the generic status ofApodanthes. It does not support a separate status of the genusBerlinianche. InRafflesiaceae, seed micromorphology is only of limited use at the species level. As far as known seed dispersal is endo- or exozoochorous in all genera.
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  • 6
    ISSN: 1617-4623
    Keywords: Rolling-circle plasmid ; Lagging strand synthesis ; Single-strand origin ; RNA polymerise Lactococcus lactis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The single-strand origin (SSO) of the rolling-circle (RC), broad-host-range lactococcal plasmid pWVO1 was functionally characterized. The activity of this SSO in the conversion of single-stranded DNA to double-stranded DNA was tested both in vivo and in vitro. In addition, the effect of this SSO on plasmid maintenance was determined. The functional pWVO1 SSO comprises a 250 by region, containing two inverted repeats (IRs). The activity of each IR was tested, separately and in combination, in a plasmid derivative that was otherwise completely devoid of structures that might function as SSO. One of the IRs (IR 1) showed some homology with other previously described SSOs of the SSOA type, as well as with the conversion signal of the Escherichia coli phage ΦX174. This IR was shown to have a partial, RNA polymerise-independent activity in complementary strand synthesis, both in vivo and in vitro. The second IR, which had no activity of its own, was required for full SSO activity, both in vivo and in vitro. The conversion of single-stranded DNA to the double-stranded form by the complete SSO was only partly sensitive to inhibition by rifampicin, indicating the existence of an RNA polymerase-independent pathway for this event. The results suggest that the pWVO1 SSO can be activated by two different routes: an RNA polymerise-dependent one (requiring the entire SSO), and an RNA polymerase-independent one (requiring only IR I).
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  • 7
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    In:  Flora Malesiana Bulletin (0071-5778) vol.27 (1974) nr.1 p.2200
    Publication Date: 2015-04-20
    Description: Dr. W. Meijer, who is Dutch-born, worked in Indonesia from 1951 to 1958, first at Bogor, then at Pajakumbuh, Sumatra, and was Forest Botanist in Sabah for several years, revisited Indonesia with a National Science Foundation travel grant under an NSF-AID (Agency for International Development) program for Scientists and Engineers in Economic Development. The University of Kentucky Research Foundation covered part of the travel costs in Indonesia together with the Regional Center for Tropical Biology (BIOTROP) in Bogor, and Weyerhaeuser Timber Co., which is now also financing the printing at U.K. of a guide on trees in Indonesia which should be an excellent tool for better training of foresters in Dendrology (tree knowledge). The Japanese Sumitomo Timber Company also acted as liaison for Dr. Meijer during his visit to East Kalimantan. Dr. Meijer has written a fully documented final report which he hopes to submit to the Indonesian government through its Academy of Science. Parts of the report will be published in the Indonesian Forestry Journal and in International Nature Conservation Journals. He hopes for continuing support from the University, its Office for International Affairs, and the U.K. Research Foundation to get this report published. Officials in the World Bank in Washington D.C. and the Smithsonian Institution have also expressed great interest in the results of Dr. Meijer’s recent mission to Indonesia. The editor is glad to print this preliminary report:
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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  • 8
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    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.30 (1984) nr.1 p.209
    Publication Date: 2015-03-06
    Description: Five new species of Rafflesia (Rafflesiaceae) are described, while attention is drawn to a sixth, possibly also new one. A key to all recognized species is given.
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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  • 9
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    In:  Flora Malesiana Bulletin (0071-5778) vol.40 (1987) nr.9/4 p.429
    Publication Date: 2015-04-20
    Description: Red merantis (Shorea subgen. Rubroshorea) are the most important commercial trees of the Dipterocarpaceae. In Sumatra at least 23 of the 55 species of Shorea belong to this group. Other groups are the Yellow merantis, White merantis, and the Meranti balau. We here give a key to the Red merantis based on characters of bark, twigs, and leaves. Some information on the distribution of the species in and outside the island has been added. We have found it difficult with the data available in the published record to make distributions as detailed as possible. Desch (1936, 1941) and Symington (1943) have divided Shorea Gaertn. into four groups based on timber and field characters, respectively. These groups were treated as subgenera by Meijer (1963), who gave the name Rubroshorea to the most well-known group of the Red Meranti. Anatomical studies by Gottwald and Parameswaran (1966) have confirmed the soundness of this classification.
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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  • 10
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    In:  Flora Malesiana - Series 1, Spermatophyta (1872-924X) vol.13 (1997) nr.1 p.1
    Publication Date: 2015-04-20
    Description: The family as here circumscribed covers the genera Mitrastema (1 species), Rafflesia (13), and Rhizanthes (2), all parasitic plants. The logo of Flora Malesiana is based on Rafflesia and is found on most of our publications. The general part of the treatment covers 10 pages and includes paragraphs on palynology by R.W.J.M. van der Ham, and on phytochemistry by R. Hegnauer. Family, genera, and Malesian species are described and annotated. Keys to the genera and species are given. Under Rafflesia a historical review of the discovery of this remarkable genus is given, and also a paragraph on ex situ cultivation. The name Rafflesia titan Jack is considered to be an incompletely known species. One new combination is formally made, on p. 23: Rafflesia arnoldii R. Brown var. atjehensis (Koord.) Meijer, comb. et stat. nov. (Basionym: Rafflesia atjehensis Koord.) Illustration is (apart from the cover photo) by 4 half-page colour photographs, 3 black/ white photographs, and 8 line drawings mostly less than full-page.
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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