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  • 1
    Electronic Resource
    Electronic Resource
    The Biology and Morphology of Crithidia acanthocephali n. sp., Leptomonas leptoglossi n. sp., and Blastocrithidia euschisti n. sp. (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three new species of Trypanosomatidae were isolated from three species of bugs: Leptomonas leptoglossi from Leptoglossus phyllopus, Crithidia acanthocephali from Acanthocephala femorata, and Blastocrithidia euschisti from Euschistus servus. All were cultured axenically and on avian embryo membranes. In addition to differences in morphology the three organisms displayed different growth rates in the chorio-allantoic fluids of duck and chick embryos incubated at 30°C. L. leptoglossi grew most abundantly. B. euschisti barely maintained itself while C. acanthocephali occupied an intermediate position.When the temperature of incubation was raised to 37°C, there was continued multiplication of L. leptoglossi and C. acanthocephali, but there was no growth of B. euschisti in either duck or chick embryos.It is suggested that the criteria of morphology, cultural characteristics in vitro and in vivo, plus physiological characters be used as future aids in classification of the Trypanosomatidae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01204.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of the Flagellar Apparatus and Attachment of Herpetomonas ampelophilae in the Gut and Malpighian Tubules of Drosophila melanogaster (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Electron microscopy was used to examine the flagellar apparatus of Herpetomonas ampelophilae from the gut and malpighian tubules of Drosophila melanogaster. The flagellates attach to the microvilli either by weaving their flagella between the microvilli or by engulfing several microvilli with an external flagellar membrane. The first type predominated in the gut while the second type was limited to the malpighian tubules. Desmosomes were not involved in either type of attachment. A subpellicular collar with emerging microtubules was found to be adjacent to the desmosome of the flagellar pocket of herpetomonads in the gut.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02854.x
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  • 3
    Electronic Resource
    Electronic Resource
    Taylor, Angela E. R. & Baker, John R. 1978. Methods of Cultivating Parasites In Vitro. Academic Press, Inc., London, New York. San Francisco. I-301 Pp. Isbn: 0-12-68555-01. Price ˜$32.00. (1980)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 27 (1980), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1980.tb04244.x
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  • 4
    Electronic Resource
    Electronic Resource
    Axenic Cultivation of Phytomonas davidi Lafont (Trypanosomatidae), a Symbiote of Laticiferous Plants (Euphorbiaceae) (1976)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Phytomonas davidi (Trypanosomatidae) originally discovered by Lafont in 1909 on the island of Mauritius was rediscovered in Euphorbia cyathophora in Florida. Successful cultures were established in diphasic medium consisting of duck blood agar and modified Phillips’medium as overlay. Optimal growth was obtained when Mansour's medium was used as overlay and poorest growth when Cowperthwaite's medium buffered at pH 5.0 was utilized for this purpose. Marked changes tending toward choanomastigotes rather than the elongate twisted promastigotes were observed in cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1976.tb03761.x
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  • 5
    Electronic Resource
    Electronic Resource
    Population Dynamics of Herpetomonas ampelophilae, with a Note on the Systematics of Herpetomonas from Drosophila spp. (1978)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 25 (1978), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Previous surveys of feral Drosophila melanogaster in Clarke County, Georgia, indicated 10 to 30% of the flies were parasitized with Herpetomonas ampelophilae. In the present study, herpetomoniasis in D. melanogaster reared in population cages rose from 0.01% to 90% in 14 days. The infection fluctuated between 5% and 100% during the 250 days in which the ageledeme was maintained. Herpetomonas were found endotrophically, peritrophically, and in the Malpighian tubules. In population cages, although there were sporadic rises in percentages, peritrophic and Malpighian tube infections usually remained low. Endotrophic infections often reached 100%, with individual flies being heavily parasitized. Although Chatton felt that several species of Herpetomonas inhabit a single species of Drosophila, we suggest that all the Herpetomonas in D. melanogaster be referred to as H. ampelophilae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1978.tb04402.x
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  • 6
    Electronic Resource
    Electronic Resource
    Growth and Reproduction of Leptomonas oncopelti in the Milkweed Bug, Oncopeltus fasciatus (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A laboratory colony of Oncopeltus fasciatus was found to be infected with Leptomonas oncopelti. Inasmuch as the parasite is transmitted from parent to offspring an opportunity presented itself to study the biology and transmission of this parasite under controlled laboratory conditions. An apparatus for observing individual bugs was designed and the presence or absence of flagellates in the feces determined. Flagellates were not shed until the bugs became adults after which they appeared in every defecation. Dissection of infected bugs revealed that flagellates were not present in the rectum until adulthood. Further studies indicated that in the midgut of the insect there is a departure from binary fission to budding. The nucleus divides and one of the newly formed nuclei migrates toward a newly formed kinetoplast. Rarely there is still another kinetoplast/nucleus division. In the event the new axoneme grows within the cytoplasmic sheath of the parent flagellum, smaller organisms produced by unequal cytokinesis remain attached. If the axoneme grows free, the smaller daughter organisms become free-swimming. Passage into the rectum of the adult bugs causes a rounding up of all parasites although the leishmaniform organisms continue to divide. It is presumed that infection of clean bugs is accomplished by the ingestion of leishmaniform organisms through a common water source. The reason for the presence of flagellates in the rectum of the adult but not in the nymphal insect and the mechanism responsible for the change from binary to unequal fission are not known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02660.x
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  • 7
    Electronic Resource
    Electronic Resource
    The Intra-erythrocytic Development of Babesiosoma stableri n. sp. in Rana pipiens pipiens (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The occurrence of Babesiosoma stableri n. sp. in the erythrocytes of Rana pipiens pipiens extends the host range of babesiosomids to the Salientia. The proposed sequence of developmental stages includes the following events: (1) two successive nuclear divisions of the uninucleate trophozoite produce a tetranucleate organism, (2) cytoplasmic cleavage initiated at each pole of the tetranucleate parasite results in four daughter individuals arranged in a cross-form, (3) the four merozoites originating from the cruciform schizont separate without the formation of a residual mass of cytoplasm, (4) gametocytes which presumably arise from differentiated merozoites do not exhibit dimorphism. The morphology and characteristics of the parasite's development were not altered when B. stableri was experimentally transferred to: R. p. sphenocephala, Rana catesbeiana, Bufo americanus, Bufo woodhousei fowleri, and Bufo terrestris. Experimental infections, with few exceptions, exhibited a peak parasitemia followed by a decline in the number of babesiosomids. Infections persisted at a low level or disappeared during the period of study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01232.x
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  • 8
    Electronic Resource
    Electronic Resource
    The Effect of Lowered Temperatures of the Host on the Reproductive Activity of Plasmodium cathemerium (1959)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 6 (1959), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Experiments were carried out to determine if the course of infection with Plasmodium cathemerium was changed when the temperature of incubation of the duck embryo host was lowered. At 30° C. the infection developed more slowly and never reached the peak seen in infected embryos incubated at 37° C. If infected embryos at 30° were returned to 37° C. the resultant parasitemia developed similarly to that usually seen in this host at the latter temperature. Studies of the periodic behavior of the parasite at 30° C. and 38° C. revealed no differences in the time of segmentation. Merozoite counts were unchanged. The reduced parasitemia in embryos at 30° C. may result from reduced ability of the parasite to penetrate new host cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1959.tb03931.x
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  • 9
    Electronic Resource
    Electronic Resource
    Biology and Structure of Phytomonas staheli sp. n., a Trypanosomatid Located in Sieve Tubes of Coconut and Oil Palms (1979)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 26 (1979), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Phytomonas staheli sp. n. is described from the oil palm (Elaeis guineensis) and the coconut palm (Cocos nucifera) of Surinam, South America. The phytomonad is the probable cause of “Hartrot” in the coconut palm and “Marchitez sopresiva” in oil palms. Parasites are confined to the sieve tubes in palms. Some success was obtained at cultivation of the organism from Elaeis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1979.tb04633.x
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  • 10
    Electronic Resource
    Electronic Resource
    Guidelines for the Description of New Species of Lower Trypanosomatids (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Morphological, cultural, and biochemical criteria that have been used in describing lower trypanosomatids, genera Blastocrithidia, Crithidia, Leptomonas, Herpetomonas, Rhynchoidomonas, and Phytomonas are reviewed. Kinetoplast structure, carbohydrate utilization, electrophoretic mobilities of isoenzymes, and kDNA fingerprinting are among the recommended criteria for species differentiation. Temperature, pH, and osmolarity tolerance are useful growth parameters. Generic placement may be assisted by the determination of nitrogenous excretion products and ornithine-arginine cycle enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1983.tb02921.x
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