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  • 1
    Electronic Resource
    Electronic Resource
    Genes from wild rice improve yield (1996)
    [s.l.] : Nature Publishing Group
    Nature 384 (1996), S. 223-224 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR — Although the wild relatives of crop species are often inferior to modern culti-vars, recent evidence suggests that they may contain genes capable of improving both the yield and quality of modern varieties1. However, these favourable genes are often masked by the effects ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/384223a0
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  • 2
    Electronic Resource
    Electronic Resource
    An active DNA transposon family in rice (2003)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 421 (2003), S. 163-167 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The publication of draft sequences for the two subspecies of Oryza sativa (rice), japonica (cv. Nipponbare) and indica (cv. 93-11), provides a unique opportunity to study the dynamics of transposable elements in this important crop plant. Here we report the use of these sequences in a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01214
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative mapping in grasses. Oat relationships (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 349-356 
    Details
    ISSN: 1617-4623
    Keywords: Wheat ; Rice ; Maize ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of RFLP linkage maps in hexaploid and diploid oat allows us to study genetic relationships of these species at the DNA level. In this report, we present the extension of a previously developed diploid oat map (Avena atlantica x A. hirtula) and its molecular-genetic relationships with wheat, rice and maize. Examination of 92–99% of the length of the oat genome map with probes common to Triticeae species, rice or maize showed that 84, 79 and 71%, respectively, was conserved between these species and oat. Generally, the orders of loci among chromosomes homoeologous to oat chromosomes A and D were the most conserved and those of chromosomes homoeologous to oat chromosome G were the least conserved. Conservation was observed for blocks ranging from whole chromosomes 101 cM long to small segments 2.5 cM long containing two loci. Comparison of the homoeologous segments of Triticeae, rice and maize relative to oat indicated that certain regions have been maintained in all four species. The relative positions of major genes governing traits such as seed storage proteins and resistance to leaf rusts have been conserved between cultivated oat and Triticeae species. Also, the locations of three vernalization/or photo-period response genes identified in hexaploid oat correspond to the locations of similar genes in homoeologous chromosomes of wheat, rice or maize. The locations of the centromeres for six of the seven oat chromosomes were estimated based on the homoeologous segments between oat and Triticeae chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290536
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative mapping in grasses. Wheat relationships (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 744-754 
    Details
    ISSN: 1617-4623
    Keywords: Oat ; Rice ; Maize ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conventionally, the genetics of species of the family Gramineae have been studied separately. Comparative mapping using DNA markers offers a method of combining the research efforts in each species. In this study, we developed consensus maps for members of the Triticeae tribe (Triticum aestivum, T. tauschii, andHordeum spp.) and compared them to rice, maize and oat. The aneuploid stocks available in wheat are invaluable for comparative mapping because almost every DNA fragment can be allocated to a chromosome arm, thus preventing erroneous conclusions about probes that could not be mapped due to a lack of polymorphism between mapping parents. The orders of the markers detected by probes mapped in rice, maize and oat were conserved for 93, 92 and 94% of the length of Triticeae consensus maps, respectively. The chromosome segments duplicated within the maize genome by ancient polyploidization events were identified by homoeology of segments from two maize chromosomes to regions of one Triticeae chromosome. Homoeologous segments conserved across Triticeae species, rice, maize, and oat can be identified for each Triticeae chromosome. Putative orthologous loci for several simply inherited and quantitatively inherited traits in Gramineae species were identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191715
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  • 5
    Electronic Resource
    Electronic Resource
    Transgressive Segregation of Tiller Angle in Rice Caused by Complementary Gene Action (1998)
    Springer
    Crop science 38 (1998), S. 12-19 
    Details
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: indica cultivars, 5002, Zhu-Fei 10, HA79317-7, and Zhen-Long 13, all having similar tiller angles. F2 populations were derived from the crosses 5002/Zhu-Fei 10 and HA79317-7/Zhen-Long 13, and divergent selection for tiller angle was practiced in each F2 population. As a result of selection, two types of true-breeding phenotypic extremes were obtained, one with larger tiller angle and the other with smaller tiller angle. For loci contributing to variation in tiller angle, the alleles of similar effect were proved to be dispersed in the original parents and associated in the extreme selection. Crossing two extreme strains derived from different original crosses revealed new transgressive segregation in the F2, demonstrating that different loci controlled tiller angle in the two cross combinations examined in this study. When the extreme phenotyes were crossed with each other, strains with the largest tiller angle represented recombinants containing all increasing alleles from the four original parents. Four quantitative trait loci (QTLs) were inferred to be responsible for the transgressive segregation in the two original crosses. The intervarietal transgression could be explained by complementary actions of additive genes that had been dispersed among the original parents. To test for allelism of the QTLs for large tiller angle and a previously identified major-gene mutant, lazy (la), one of the extreme strains from the second cycle of selection having the largest tiller angle was crossed with the lazy growth mutant, H-79. Results indicated that la was not allelic to any of the QTLs. Breeding experiments demonstrated that multiple crossing followed by one cycle of selection could be used to accumulate into one strain the genes that were dispersed in the parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/
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  • 6
    Electronic Resource
    Electronic Resource
    Rice PHYC gene: structure, expression, map position and evolution (2000)
    Springer
    Plant molecular biology 44 (2000), S. 27-42 
    Details
    ISSN: 1573-5028
    Keywords: gene structure ; genomic mapping ; phylogeny ; phytochrome genes ; rice ; transcription start site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although sequences representing members of the phytochrome (phy) family of photoreceptors have been reported in numerous species across the phylogenetic spectrum, relatively few phytochrome genes (PHY) have been fully characterized. Using rice, we have cloned and characterized the first PHYC gene from a monocot. Comparison of genomic and cDNA PHYC sequences shows that the rice PHYC gene contains three introns in the protein-coding region typical of most angiosperm PHY genes, in contrast to Arabidopsis PHYC, which lacks the third intron. Mapping of the transcription start site and 5′-untranslated region of the rice PHYC transcript indicates that it contains an unusually long, intronless, 5′-untranslated leader sequence of 715 bp. PHYC mRNA levels are relatively low compared to PHYA and PHYB mRNAs in rice seedlings, and are similar in dark- and light-treated seedlings, suggesting relatively low constitutive expression. Genomic mapping shows that the PHYA, PHYB, and PHYC genes are all located on chromosome 3 of rice, in synteny with these genes in linkage group C (sometimes referred to as linkage group A) of sorghum. Phylogenetic analysis indicates that rice phyC is closely related to sorghum phyC, but relatively strongly divergent from Arabidopsis phyC, the only full-length dicot phyC sequence available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006488119301
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.) (2000)
    Springer
    Euphytica 116 (2000), S. 17-22 
    Details
    ISSN: 1573-5060
    Keywords: Blast (Pyricularia grisea Sacc.) ; disease resistance ; microsatellite ; RFLP (Restrictionfragment length polymorphism) ; rice (Oryza sativa L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A single dominant blast resistance gene conferring resistance to a Korean rice blast isolate was identified in rice variety `Suweon 365'. We report the chromosomal localization and molecular mapping of this blast resistance gene designated as Pi-18, which confers resistance to Korean isolate `KI-313' of the blast pathogen. To know whether there is a relationship among genes conditioning resistance to location-specific isolates of the blast pathogen and thereby to identify linked markers to resistance gene for isolate KI-313 collected in Korea, RFLP markers previously reported to be linked to major blast resistance genes in different rice germplasm and other markers mapped to nearby regions were surveyed for polymorphism between a resistant (`Suweon 365') and a susceptible (`Chucheongbyeo') parent. Linkage associations of the RFLP markers with the resistance gene were verified using an F2 and F3 segregating population of known blast reaction. RFLP analysis showed that Pi-18 was located near the end of chromosome 11, linked to a single copy clone RZ536 at a distance of 5.4 centiMorgans (cM) and that this gene was different from Pi-1(t). An allelism test revealed that this gene was also different from Pi-k. Currently, a combination of RAPD and microsatellite primers is being employed to find additional markers in this region. Tightly linked DNA markers will facilitate selection for resistant genotypes in breeding programs and provide the basis for map based cloning of this new blast resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004045812357
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  • 8
    Electronic Resource
    Electronic Resource
    Microsatellite marker development, mapping and applications in rice genetics and breeding (1997)
    Springer
    Plant molecular biology 35 (1997), S. 89-99 
    Details
    ISSN: 1573-5028
    Keywords: allelic diversity ; microsatellite marker ; molecular mapping ; polymerase chain reaction (PCR) ; simple sequence length polymorphism (SSLP) ; simple sequence repeat (SSR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microsatellites are simple, tandemly repeated di- to tetra-nucleotide sequence motifs flanked by unique sequences. They are valuable as genetic markers because they are co-dominant, detect high levels of allelic diversity, and are easily and economically assayed by the polymerase chain reaction (PCR). Results from screening a rice genomic library suggest that there are an estimated 5700-10 000 microsatellites in rice, with the relative frequency of different repeats decreasing with increasing size of the motif. A map consisting of 120 microsatellite markers demonstrates that they are well distributed throughout the 12 chromosomes of rice. Five multiple copy primer sequences have been identified that could be mapped to independent chromosomal locations. The current level of genome coverage provided by these simple sequence length polymorphisms (SSLPs) in rice is sufficient to be useful for genotype identification, gene and quantitative trait locus (QTL) analysis, screening of large insert libraries, and marker-assisted selection in breeding. Studies of allelic diversity have documented up to 25 alleles at a single locus in cultivated rice germplasm and provide evidence that amplification in wild relatives of Oryza sativa is generally reliable. The availability of increasing numbers of mapped SSLP markers can be expected to complement existing RFLP and AFLP maps, increasing the power and resolution of genome analysis in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005711431474
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  • 9
    Electronic Resource
    Electronic Resource
    Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers (1995)
    Springer
    Molecular breeding 1 (1995), S. 375-387 
    Details
    ISSN: 1572-9788
    Keywords: disease resistance ; gene combination ; Oryza sativa L. ; RAPD ; RFLP ; Xanthomonas oryzae pv.oryzae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four genes of rice,Oryza sativa L., conditioning resistance to the bacterial blight pathogenXanthomonas oryzae pv.oryzae (X. o. pv.oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed betweenxa-5 and RFLP marker lociRZ390, RG556 orRG207 on chromosome 5.Xa-3 andXa-4 were linked to RFLP locusXNpb181 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker toXa-10, also located on chromosome 11, was the RAPD locusO07 2000 at a distance of 5.3 cM. From this study, the conventional map [19, 28] and two RFLP linkage maps of chromosome 11 [14, 26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes,Xa-4 +xa-5 andXa-4 +Xa-10. Lines carryingXa-4 +xa-5 andXa-4 +Xa-10 were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs of genes were resistant to more of the isolates than their single-gene parental lines. Lines carryingXa-4 +xa-5 were more resistant to isolates of race 4 than were either of the parental lines (‘quantitative complementation’). No such effects were seen forXa-4 +Xa-10. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01248415
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  • 10
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    A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice (2020)
    National Academy of Sciences
    Details
    Publication Date: 2020-05-12
    Description: Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we developPCR-mediatedLinkage of barcodedAdaptersTo nucleic acidElements forsequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome forOryza sativacovering 2,300 genes and constructing a high-quality protein–protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein–protein interactions in a wide range of organisms.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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