ISSN:
1432-0983
Keywords:
Lower plant mitochondrial DNA
;
Cytochrome oxidase subunit III gene
;
Moss (Physcomitrella patens)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The first mitochondrial-encoded gene of an archeogoniate has been identified, cloned and sequenced. The cytochrome oxidase III gene (cox3) of the moss Physcomitrella patens consists of a 618 bp open reading frame with high homology (around 72%) to known cox3 sequences of higher plants. Nevertheless, it is a quarter shorter than these. The cox3 gene of P. patens contains no introns and reveals a G+C-content of 41.3%. The region containing the cox3 gene exists as a single copy in the mitochondrial genome as shown by restriction mapping. In the 5′ flanking sequence a putative ribosome binding site and a putative secondary structure were found. Two main transcripts of 2.4 kb and 2.6 kb were detected indicating a complex mitochondrial transcription pattern possibly due to co-transcription. Additional open reading frames were found downstream from, as well as upstream of, the cox3 gene. In Western blots a polyclonal cox3 antibody from yeast detected one single band with an apparent molecular weight of 22 kDa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00318522
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