Publication Date:
2015-08-01
Description:
Metagenomic sequencing increased our understanding of the role of the microbiome in health and disease, yet it only provides a snapshot of a highly dynamic ecosystem. Here, we show that the pattern of metagenomic sequencing read coverage for different microbial genomes contains a single trough and a single peak, the latter coinciding with the bacterial origin of replication. Furthermore, the ratio of sequencing coverage between the peak and trough provides a quantitative measure of a species' growth rate. We demonstrate this in vitro and in vivo, under different growth conditions, and in complex bacterial communities. For several bacterial species, peak-to-trough coverage ratios, but not relative abundances, correlated with the manifestation of inflammatory bowel disease and type II diabetes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Korem, Tal -- Zeevi, David -- Suez, Jotham -- Weinberger, Adina -- Avnit-Sagi, Tali -- Pompan-Lotan, Maya -- Matot, Elad -- Jona, Ghil -- Harmelin, Alon -- Cohen, Nadav -- Sirota-Madi, Alexandra -- Thaiss, Christoph A -- Pevsner-Fischer, Meirav -- Sorek, Rotem -- Xavier, Ramnik J -- Elinav, Eran -- Segal, Eran -- New York, N.Y. -- Science. 2015 Sep 4;349(6252):1101-6. doi: 10.1126/science.aac4812. Epub 2015 Jul 30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. ; Immunology Department, Weizmann Institute of Science, Rehovot, Israel. ; Department of Biological services, Weizmann Institute of Science, Rehovot, Israel. ; Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, Israel. ; Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School and Broad Institute, Cambridge, MA, USA. ; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel. ; Immunology Department, Weizmann Institute of Science, Rehovot, Israel. eran.elinav@weizmann.ac.il eran.segal@weizmann.ac.il. ; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. eran.elinav@weizmann.ac.il eran.segal@weizmann.ac.il.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26229116" target="_blank"〉PubMed〈/a〉
Keywords:
Bacteria/classification/genetics/*growth & development
;
Diabetes Mellitus, Type 2/*microbiology
;
Gastrointestinal Tract/*microbiology
;
Genome, Bacterial
;
Humans
;
Inflammatory Bowel Diseases/*microbiology
;
Metagenome
;
Metagenomics
;
Microbiota/genetics/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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