Publication Date:
2013-10-11
Description:
Cyanobacteria are photosynthetic organisms responsible for approximately 25% of organic carbon fixation on the Earth. These bacteria began to convert solar energy and carbon dioxide into bioenergy and oxygen more than two billion years ago. Cyanophages, which infect these bacteria, have an important role in regulating the marine ecosystem by controlling cyanobacteria community organization and mediating lateral gene transfer. Here we visualize the maturation process of cyanophage Syn5 inside its host cell, Synechococcus, using Zernike phase contrast electron cryo-tomography (cryoET). This imaging modality yields dramatic enhancement of image contrast over conventional cryoET and thus facilitates the direct identification of subcellular components, including thylakoid membranes, carboxysomes and polyribosomes, as well as phages, inside the congested cytosol of the infected cell. By correlating the structural features and relative abundance of viral progeny within cells at different stages of infection, we identify distinct Syn5 assembly intermediates. Our results indicate that the procapsid releases scaffolding proteins and expands its volume at an early stage of genome packaging. Later in the assembly process, we detected full particles with a tail either with or without an additional horn. The morphogenetic pathway we describe here is highly conserved and was probably established long before that of double-stranded DNA viruses infecting more complex organisms.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984937/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984937/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dai, Wei -- Fu, Caroline -- Raytcheva, Desislava -- Flanagan, John -- Khant, Htet A -- Liu, Xiangan -- Rochat, Ryan H -- Haase-Pettingell, Cameron -- Piret, Jacqueline -- Ludtke, Steve J -- Nagayama, Kuniaki -- Schmid, Michael F -- King, Jonathan A -- Chiu, Wah -- AI0175208/AI/NIAID NIH HHS/ -- GM080139/GM/NIGMS NIH HHS/ -- P41 GM103832/GM/NIGMS NIH HHS/ -- P41GM123832/GM/NIGMS NIH HHS/ -- PN2 EY016525/EY/NEI NIH HHS/ -- PN2EY016525/EY/NEI NIH HHS/ -- R01 GM080139/GM/NIGMS NIH HHS/ -- R56 AI075208/AI/NIAID NIH HHS/ -- T15 LM007093/LM/NLM NIH HHS/ -- T15LM007093/LM/NLM NIH HHS/ -- T32GM007330/GM/NIGMS NIH HHS/ -- England -- Nature. 2013 Oct 31;502(7473):707-10. doi: 10.1038/nature12604. Epub 2013 Oct 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Center for Macromolecular Imaging, Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24107993" target="_blank"〉PubMed〈/a〉
Keywords:
Aquatic Organisms/cytology/ultrastructure/virology
;
Bacteriophages/*growth & development/*ultrastructure
;
Cryoelectron Microscopy/*methods
;
Electron Microscope Tomography/*methods
;
Models, Biological
;
Synechococcus/cytology/*ultrastructure/*virology
;
*Virus Assembly
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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