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  • 1
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    Stable, monomeric variants of lambda Cro obtained by insertion of a designed beta-hairpin sequence (1990)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1990-12-21
    Description: lambda Cro is a dimeric DNA binding protein. Random mutagenesis and a selection for Cro activity have been used to identify the contacts between Cro subunits that are crucial for maintenance of a stably folded structure. To obtain equivalent contacts in a monomeric system, a Cro variant was designed and constructed in which the antiparallel beta-ribbon that forms the dimer interface was replaced by a beta-hairpin. The engineered monomer has a folded structure similar to wild type, is significantly more stable than wild type, and exhibits novel half-operator binding activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mossing, M C -- Sauer, R T -- AI-16982/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1990 Dec 21;250(4988):1712-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2148648" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Bacteriophage lambda/*genetics ; Circular Dichroism ; *DNA-Binding Proteins ; Escherichia coli/genetics ; *Genetic Variation ; Macromolecular Substances ; Models, Molecular ; Molecular Sequence Data ; Mutagenesis ; Protein Conformation ; Repressor Proteins/*genetics/metabolism ; Thermodynamics ; Transcription Factors/*genetics ; Viral Proteins ; Viral Regulatory and Accessory Proteins
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Upstream operators enhance repression of the lac promoter (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-08-22
    Description: To study regulation of transcription by distant elements, a wild-type lac operator was inserted upstream of a promoter-constitutive operator control region. The upstream operator is shown to aid in repression of transcription from the mutant control region. The effectiveness of the upstream operator as a function of its distance from the mutant control region parallels the length dependence observed for DNA cyclization. A quantitative model is proposed for action-at-a-distance of DNA control sites in which protein-protein and protein-DNA interactions are mediated by DNA looping. In this model, the effective concentrations of interacting proteins that are tethered by DNA are determined by the length of the intervening DNA and by its inherent bending and torsional stiffness. This model makes a number of predictions for both eukaryotic and prokaryotic control sequences located far from their sites of action.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mossing, M C -- Record, M T Jr -- GM23467/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1986 Aug 22;233(4766):889-92.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3090685" target="_blank"〉PubMed〈/a〉
    Keywords: Galactokinase/metabolism ; *Gene Expression Regulation ; *Lac Operon ; Models, Genetic ; *Promoter Regions, Genetic ; Repressor Proteins ; beta-Galactosidase/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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