ISSN:
1573-4986
Keywords:
N-acetylneuraminic acid cytidylyltransferase
;
rat
;
NeuAc or sialic acid, N-acetyl-D-neuraminic acid
;
CTP, Cytidine 5′-triphosphate
;
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis
;
PSA, polysialic acid
;
N-CAM, Neural cell adhesion molecules
;
CMP-Neu5Ac, cytidine 5′-monophospho-N-acetyl neuraminic acid and DTT, dithiothreitol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract We report the postnatal developmental profiles of N-acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-Neu5Ac synthetase) in different rat tissues. This enzyme, which catalyses the activation of NeuAc to CMP-Neu5Ac, was detected in brain, kidney, heart, spleen, liver, stomach, intestine, lung, thymus, prostate and urinary bladder but not in skeletal muscle. Comparative analysis of the different specific activity profiles obtained shows that the expression of CMP Neu5Ac synthetase is tissue-dependent and does not seem to be embryologically determined. Changes in the level of sialylation during development were also found to be intimately related to variations in the expression of this enzyme, at least in brain, heart, kidney, stomach, intestine and lung.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006940927639
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