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  • 1
    Electronic Resource
    Electronic Resource
    Evolution of thedec-1 eggshell locus inDrosophila. I. Restriction site mapping and limited sequence comparison in themelanogaster species subgroup (1991)
    Springer
    Journal of molecular evolution 33 (1991), S. 321-331 
    Details
    ISSN: 1432-1432
    Keywords: Drosophila ; dec-1 locus ; Restriction map conservation ; Sequence comparison ; Melanogaster species subgroup ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have analyzed ∼18 kb of DNA in and upstream of thedefective chorion-1 (dec-1) locus of the eight known species of themelanogaster species subgroup ofDrosophila. The restriction maps ofD. simulans, D. mauritiana, D. sechellia, D. erecta, andD. orena are shown to have basically the restriction map ofD. melanogaster, whereas the maps ofD. teissieri andD. yakuba were more difficult to align. However, the basic amount of DNA and sequence arrangement appear to have been conserved in these species. A small deletion of varying length (65–200 bp) is found in a repeated sequence of the central transcribed region ofD. melanogaster, D. simulans, andD. erecta. Restriction site mapping indicated that thedec-1 gene is highly conserved in themelanogaster species subgroup. However, sequence comparison revealed that the amount of nucleotide and amino acid substitution in the repeated region is much larger than in the 5′ translated region. The 5′ flanking region showed noticeable restriction site polymorphisms between species. Based on calculations from the restriction maps a dendrogram was derived that supports earlier published phylogenetic relationships within themelanogaster species subgroup except that theerecta-orena pair is placed closer to themelanogaster complex than toD. teissieri andD. yakuba.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02102863
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  • 2
    Electronic Resource
    Electronic Resource
    Evolution of thedec-1 eggshell locus inDrosophila. II. Intraspecific DNA sequence analysis reveals length mutations in a repetitive region inD. melanogaster (1993)
    Springer
    Journal of molecular evolution 36 (1993), S. 536-544 
    Details
    ISSN: 1432-1432
    Keywords: Drosophila ; dec-1 eggshell gene ; Wild-type variants ; Repeated region ; DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thedec-1 eggshell gene inDrosophila melanogaster encodes follicle cell proteins required for proper eggshell assembly. As shown by Southern and Northern analyses thedec-1 gene occurs in four alleles (Fcl-4) among wild-type strains. Its second exon has a distinct feature in the form of 12 repeats with 78–91 nucleotides; the first five show nearly 100% homology. DNA sequence comparison of the repeated region of the alleles revealed that the length polymorphisms are caused by changes in the numbers of the first five repeats. The results suggest that the alleles have been generated by unequal intragenic crossing-over and/or slippage during DNA replication and that the allelic length variants have arisen independently. The possiblilty that the most common allele,FC1, has a selective advantage over the other alleles is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00556358
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  • 3
    Electronic Resource
    Electronic Resource
    Ecdysterone responsive functions in the mutantl(1)su(f) ts67g ofDrosophila melanogaster (1984)
    Springer
    Development genes and evolution 193 (1984), S. 48-51 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila development ; Ts mutant ; Ecdysterone ; Salivary glands ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transferring the temperature sensitive mutantl(1)su(f) ts67g from 25° C to 30° C before or early in the third larval instar blocks the increase in the ecdysterone titer that normally occurs at the end of the larval period. Feeding exogenous ecdysterone to these hormone-deficient larvae results in the formation of pseudopupae. The mutant was used to study ecdysterone-inducible functions in late larval salivary glands by preparing three animal samples with different hormone titers: the titer was low in one sample because of an earlier temperature shift, high in a second sample because the larvae were subsequently transferred to ecdysterone-supplemented food, and also high in a third sample that was kept at 25°C, providing a control for normal development. The effect of the different hormone conditions was studied by35S-methionine labeling of the salivary gland proteins during the larval to prepupal transition and the prepupal period. The results indicate that synthesis of several of the proteins normally appearing during the transition and prepupal period is induced by exogenous ecdysterone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848600
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of the l(1)su(f)ts67g mutation ofDrosophila melanogaster on glue protein synthesis (1981)
    Springer
    Development genes and evolution 190 (1981), S. 308-312 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; ts-Suppressor mutant ; Glue proteins ; Intermolt puffs ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The l(1)su(f)ts67g mutation has been shown to suppress the developmentally regulated expression of glue protein genes at 30°C. Transferring mutant larvae to the restrictive temperature before the end of the second larval instar results in the absence or extreme reduction of glue protein synthesis while general protein synthesis is unaffected. At the same time, the three glue protein correlated chromosomal regions 3C, 25B, and 68C continue to show prominent puffs. The results suggest that the mutation may be affecting the processing or translatability of specific mRNAs rather than the translational machinery itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00863267
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  • 5
    Electronic Resource
    Electronic Resource
    Stage specific synthesis of some follicle cell proteins inDrosophila melanogaster (1985)
    Springer
    Development genes and evolution 194 (1985), S. 436-439 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila oogenesis ; Protein synthesis ; SDS-gel electrophoresis ; Temperature-sensitive mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ovarian protein synthesis in the temperature-sensitive mutantl(1)su(f) ts67g was analysed at the permissive and non-permissive temperature by SDS-polyacrylamide gel electrophoresis of35S-methionine labelled ovaries. The synthesis of yolk and three other ovarian proteins of approximative molecular weights of 92K, 82K and 76K, respectively, were affected by the shift to the restrictive temperature. Examination of protein synthesis pattern in staged egg chambers revealed that these three proteins were synthesized at stage 10. Analysis of separated cell types present at stage 10 demonstrate that the three proteins were follicle cell products. We have been unable to identify these proteins as any previously described follicle cell proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848559
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  • 6
    Electronic Resource
    Electronic Resource
    The role of su(f) gene function and ecdysterone in transcription of glue polypeptide mRNAs in Drosophila melanogaster (1983)
    Springer
    Molecular genetics and genomics 192 (1983), S. 395-401 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of the l(1)su(f) ts67g mutation and ecdysterone on the expression of glue polypeptide genes in Drosophila melanogaster has been studied. Using cloned DNA harboring glue protein genes Sgs3, Sgs4, Sgs7, and Sgs8, and Northern blot hybridization we show that no or extremely reduced accumulation of RNAs from these genes occurs in mutant larvae shifted to 30°C before and through 70 h after oviposition. On the other hand, a shift-up at 75 h or later results in the production of nearly normal amounts of these RNAs 48 h after shift-up. The results indicate functions of the su(f) gene other than suppression of forked bristles, namely that it is essential early in the third instar for the later transcription of the glue polypeptide genes. We also show that administration of exogenous ecdysterone to hormone deficient larvae induces transcription of Sgs3, Sgs4, Sgs7, and Sgs8 RNAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392181
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  • 7
    Electronic Resource
    Electronic Resource
    The ribosomal proteins of Drosophila melanogaster (1974)
    Springer
    Molecular genetics and genomics 128 (1974), S. 241-247 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ribosomes from mid- and late-third instar larvae, prepupae, 24-hours old pupae, and adult flies were isolated and washed with 0.5 M NH4Cl, 1mM Mg++. The ribosomal proteins were extracted and analyzed by two-dimensional polyacrylamide gel electrophoresis. Defined differences are shown between the protein patterns of whole ribosomes from mid-third instar larvae on one hand and prepupae, 24-hours old pupae, and imagos on the other hand. Late-third instar larval ribosomes exhibit an intermediate protein pattern suggesting a transitory stage with both unchanged and changed ribosomes in larvae ready to pupate. Apart form 1–3 major proteins the alterations comprise medium and minor spots. It cannot be excluded, however, that changes involving weak spots may be attributed to supernatant proteins, which, despite the high salt wash, bind strongly to the ribosomes during preparation. The number of proteins of whole ribosomes from D. melanogaster is estimated to be 68–69.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267113
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  • 8
    Electronic Resource
    Electronic Resource
    Imaginal disc ribosomal proteins of D. melanogaster (1978)
    Springer
    Molecular genetics and genomics 159 (1978), S. 85-87 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ribosomal proteins from undifferentiated imaginal discs of Drosophila melanogaster were analyzed by two-dimensional gel electrophoresis and compared with the ribosomal protein pattern of adult flies. It is shown that the ribosomal proteins from these discs are qualitatively identical with those of adult flies except that two acidic proteins are missing in the discs. This heterogeneity is discussed in terms of the functional roles these two proteins may carry in connection with disc differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401751
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic localization of a follicle cell protein locus in Drosophila melanogaster (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 375-378 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three variant forms of a “novel” set of follicle cell proteins (Fc) were found when screening geographic wild-type strains of Drosophila melanogaster by SDS-polyacrylamide gel electrophoresis of 35S-methionine labelled ovaries. These variant forms were used to establish X chromosomal linkage and for further genetic localization by both recombinant analysis and by cytogenetical mapping. A locus involved in the synthesis of Fc proteins was localized to the 7C1-9 region, i.e. very close to the singed locus (21.0 cM). The number of Fc proteins, their variation and possible function is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331326
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  • 10
    Electronic Resource
    Electronic Resource
    The ribosomal proteins of Drosophila melanogaster (1970)
    Springer
    Molecular genetics and genomics 108 (1970), S. 349-357 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ribosomes were isolated from larvae and adult flies, and the purity of the preparation was checked by electron microscopy. The ribosomal proteins were extracted with cold dilute hydrochloric acid, and precipitated with cold acetone. The proteins were characterized by polyacrylamide gel electrophoresis. At pH 3.0 at least 25 bands of different color intensities were resolved, forming a complex pattern. On the basis of electrophoretic mobilities, it was shown that some ribosomal proteins are species-specific, and that larval ribosomes have three protein components more than ribosomes from adult flies. Incubation of the ribosomes with 0.75 M NH4Cl at a low Mg++ concentration lead to detachment of 64% of the ribosomal protein. This detachment of protein molecules was considerably reduced by a five-fold increase of Mg++ ions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267772
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