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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 53 (2004), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A total of 45 field sites in England were surveyed once for the presence of apothecia of Tapesia yallundae from 1992 to 1994. Apothecia were found at 21 locations and were mainly present on less than 3% of stems. However, apothecia were found on 1532% of stems at four sites. Analysis of the growth characteristics of ascospore isolates from seven sites showed that most produced colonies characteristic of the W-type of T. yallundae, with only one site yielding the R-type, Most ascospore isolates were resistant to the fungicide benomyl and effectively all remained sensitive to prochloraz. The results of the study are discussed in relation to the fungicidal control and epidemiology of T. yallundae, and the risk of spread of disease from set-aside sites.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 42 (1993), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Bacteria isolated from wheat seedlings, plants or straw from several field sites were screened for antagonism towards the cereal eyespot pathogen Pseudocercosporella herpotrichoides on several media of differing nutrient status. Thirteen out of 348 isolates inhibited pathogen growth on low-nutrient media and several also prevented spore germination or reduced germ tube extension. These were selected for further tests on wheat seedlings inoculated with the eyespot fungus. Twelve known bacterial antagonists of other fungal plant pathogens were tested in vitro using the same methods, and the majority showed some activity towards P. herpotrichoides. Selected isolates were equally inhibitory to both W and R pathotypes of the fungus. Effects of potential antagonists on disease development were assessed by scoring lesions or by counting the number of infection plaques formed by the fungus on leaf sheaths. Two isolates of Pseudomonas fluorescens, along with a commercial strain of Streptomyces griseoviridis, showed activity both in vitro and in subsequent infection trials with plants and may therefore be of potential value as antagonists of P. herpotrichoides.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 42 (1993), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The eyespot fungus Pseudocercosporella herpotrichoides survives on straw colonized during infection of a previous cereal crop, which then serves as an inoculum source for subsequent infection cycles. Tests were devised to identify fungal antagonists capable of competing with the pathogen on straw, and suppressing inoculum production and host infection. The 228 fungal isolates from wheat seedlings, plants or straw were screened for activity, along with 13 fungi and a commercial strain of Streptomyces griseoviridis which had proven biocontrol activity against other fungal pathogens. Potential antagonists were selected on the basis of inhibition or overgrowth of P. herpotrichoides on several contrasting nutrient media. Co-inoculation of straw with the pathogen and test antagonists reduced disease severity in pot trials using this straw as an inoculum source. When straw pre-inoculated with the pathogen was used, fewer antagonists proved effective in suppressing eyespot disease. However, one isolate, a Trichoderma sp., gave positive results both in vitro and in infection trials using different inoculation procedures, and may therefore be an effective antagonist of P. herpotrichoides during the saprophytic survival phase of the pathogen life cycle.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 39 (1990), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The host ranges of 33 isolates of the downy mildew fungus Peronospora parasitica from different Brassica hosts and diverse geographic origins were assessed on a standard set of Brassica accessions Isolates from each host species had a distinct host range, and in some cases isolates from the same host species but different geographic origins differed in host range. Most isolates were capable of infecting hosts other than their species of origin. These results are discussed in relation to species specificity and the conservation of certain virulence gene combinations. The epidemiological implications for cross-infection of Brassica hosts by P. parasitica isolates in the field are also considered.
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  • 7
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Pretreatment of the first true leaves of oilseed rape plants (Brassica napus cv. Bristol) with menadione sodium bisulphite (MSB) locally and systemically induced resistance, as shown by reduced lesion size and number, to infection by the fungal pathogen Leptosphaeria maculans, the causal agent of stem canker. Using a known systemic activator of salicylic acid-dependent PR-1 induction, acibenzolar-S-methyl (BTH; S-methylbenzo[1,2,3]thiadiazole-7-carbothiate) as a comparison, real-time PCR expression analysis of genes encoding a pathogenesis-related protein 1 (PR-1) and an ascorbate peroxidase (APX) demonstrated a systemic enhancement of APX expression in MSB-pretreated plants, with no effect on PR-1 expression, suggesting augmented reactive oxygen species production in MSB-pretreated plants. The results demonstrate MSB to be an effective resistance activator in oilseed rape, and potentially useful for the control of stem canker.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 43 (1994), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Apothecia of Tapesia yallundae were collected from a set-aside straw stubble site in Lincolnshire in March 1993. Single ascospore isolates were obtained which produced colonies with morphologies and growth rates characteristic of the R-pathotype of Pseudocercosporella herpotrichoides. Isolates were confirmed to be R-type by the use of randomly amplified polymorphic DNA (RAPD) markers. These observations confirm that T. yallundae is the teleomorph of the R-type of P. herpotrichoides and represent the first detection of the sexual stage of the R-type in the UK.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The production of viable oospores of Peronospora parasitica under laboratory conditions and the recovery of isolates (referred to as sexual progeny) from these oospore populations are described. Oospores were produced when isolates of opposite sexual compatibility type, specialized to the same or different Brassica species, were grown together in seedling cotyledons of a host line capable of supporting growth of both isolates. Recovery of sexual progeny from oospore populations produced from two out of four pairings between isolates specialized to the same host species (homologous pairings) proved relatively easy. On the basis of their characterization with respect to virulence, response to phenylamide fungicides, sexual compatibility type and isoenzyme polymorphisms, there was evidence that the sexual progeny from these homologous pairings could be of hybrid origin. For the first time in a member of the Peronosporaceae, it proved possible to recover and successfully characterize a few sexual progeny from pairings between isolates specialized to different host species (heterologous pairings). However, the majority of such isolates sporulated weakly and as a consequence proved difficult to maintain and were lost. Nevertheless, some evidence for the hybrid nature of progeny from heterologous pairings was obtained.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 39 (1990), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Pseudocercosporella herpotrichoides, causal agent of the eyespot disease of cereals, are usually classified as W-type or R-type on the basis of differential pathogenicity towards wheat and rye hosts, a distinction often correlated with cultural morphology. Polyacrylamide gel electrophoresis (PAGE) of soluble proteins was used to compare 101 isolates of Pseudocercosporella spp. including both W-types and R-types, and the related species P. anguioides and P. aestiva. Of 16 enzyme activities detected, seven were differential for the pathotypes. Isozyme polymorphisms for five enzymes clearly differentiated W-type from R-type isolates, a result confirmed by pathogenicity tests. Within each pathotype, isozyme patterns were similar for isolates of diverse geographical origins, including Europe, North America, South Africa and New Zealand. Isolates of P. anguioides and P. aestiva could also be distinguished on the basis of isozyme patterns. The mechanisms maintaining these polymorphisms, and the value of PAGE for population studies of this pathogen, are discussed.
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