Publication Date:
2022-05-25
Description:
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Experimental Parasitology 113 (2006): 130-133, doi:10.1016/j.exppara.2005.12.013.
Description:
Nitric oxide (NO) is synthesized enzymatically by nitric oxide synthase (NOS). Several
groups have previously presented evidence for NOS activity and immunoreactivity in several
parasitic platyhelminths, including schistosomes. Here, we use 4,5-diaminofluorescein-2
diacetate (DAF-2 DA), a fluorescent indicator of NO, to detect NO in living schistosomes. In
adult worms, DAF-2 fluorescence is found selectively in epithelial-like cells. Fluorescence
increases when worms are incubated in L-arginine, the precursor of NO synthesis, and decreases
dramatically in the presence of the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME),
indicating that predicted NO release may be NOS-dependent, and that enzymatic NO signaling
pathways may play an important role in schistosome physiology.
Description:
This work was
supported by NIH grant NS 39103 and NSF grants 0304569 (LLM), and NIH grant AI 40522
and the Neal Cornell Research Fund at the Marine Biological Laboratory (RMG).
Keywords:
Nitric oxide
;
Schistosomiasis
;
Trematode
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
175555 bytes
Format:
application/pdf
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