ISSN:
0018-019X
Keywords:
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The specific attachment of bathophenanthroline-ruthenium(II) complexes as non-radioactive label molecules to synthetically 5′-NH2-modified oligonucleotides is described. After excitation by light pulses, the fluorescence of these labels can be measured by a time-resolved mode woth high sensitivity. No quenching takes place due to coupling of the Ru complexes to the DNA. Ru-complex-labelled oligonucleotides still hybridize specifically to complementary DNA sequences, and no quenching is observed in the course of the hybridization process.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/hlca.19880710826
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