ISSN:
1040-0397
Keywords:
Biosensor
;
Hydrogen peroxide
;
Creatinine
;
Creatine
;
Peroxidase
;
Sarcosine oxidase
;
Creatine amidinohydrolase
;
Creatinine amidohydrolase
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Peroxidase (POD) was entrapped on a ferrocene (Fc)-embedded carbon paste (CP) electrode with a dialysis membrane to construct a POD-Fc-CP electrode for the reductive amperometric determination of hydrogen peroxide (H2O2). The electrode allowed the H2O2 detection down to 10 nM (under stirring), with a linear relation up to 15 μM, and without change of the response over one month. On the other hand, a plastic-formed carbon (PFC) electrode can donate electrons to POD without any specific mediator. The POD-entrapped PFC electrode produced a relative current density of 24% compared with the POD-Fc-CP electrode. The reductive detection of H2O2 with the POD-Fc-CP electrode was applied to the determination of creatinine and creatine in serum and urine using sarcosine oxidase, creatine amidinohydrolase, and creatinine amidohydrolase as the reagents. The significance of the membrane-covered POD-Fc-CP electrode in the coupling of the oxidase reaction is also discussed.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140091603
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