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    Insertion of a new gene of viral origin into bone marrow cells of mice (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-05-30
    Description: DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a tk++ status in vitro using methotrexate as a selective agent. HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mercola, K E -- Stang, H D -- Browne, J -- Salser, W -- Cline, M J -- New York, N.Y. -- Science. 1980 May 30;208(4447):1033-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6246577" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Bone Marrow/*enzymology ; Bone Marrow Transplantation ; DNA, Viral/analysis ; Drug Resistance ; *Genes, Viral ; L Cells (Cell Line) ; Methotrexate/pharmacology ; Mice ; Simplexvirus/enzymology/*genetics ; Species Specificity ; Thymidine Kinase/*genetics ; *Transformation, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of a methotrexate-resistant dihydrofolate reductase gene by transformed hematopoietic cells of mice (1983)
    Springer
    Somatic cell and molecular genetics 9 (1983), S. 55-67 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract DNA-mediated gene transfer was used to introduce DNA from a methotrexate-resistant mouse fibroblast cell line into mouse bone marrow cells. This cell line contained a methotrexate-resistant dihydrofolate reductase, active at 10−4 M methotrexate, which was electrophoretically separable from the wild-type mouse enzyme. Transformed hematopoietic cells were returned to irradiated mice and selected in vivo by methotrexate administration. Some recipients of transformed marrow cells expressed the electrophoretically distinct, methotrexate-resistant dihydrofolate reductase in hematopoietic cells. These observations suggest that successful transformation of marrow stem cells to methotrexate resistance is accomplished by insertion of a dihydrofolate reductase gene coding for a mutant enzyme that is highly resistant to methotrexate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01544048
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    Paper (German National Licenses)
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