ISSN:
1573-9058
Keywords:
allophycocyanin
;
dithiothreitol
;
energy transfer
;
N-succinimidyl-3-(2-pyridyldithio)propionate
;
phycocyanin
;
phycoerythrocyanin
;
rod-core complex (αβ)6 PCLRC 27 (αβ)3 APCLC 8.9
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The model conjugates phycocyanin-allophycocyanin (C-PC-APC) and phycoerythrocyanin-phycocyanin-allophycocyanin (PEC-C-PC-APC) were synthesized by using a heterobifunctional coupling reagent N-succinimidyl-3-(2-pyridyldithio)propionate. The rod-core complex (αβ)6 PCLRC 27(αβ)3 APCLC 8.9 and phycobilisomes were separated from Anabaena variabilis. Energy transfer features for the conjugates and the complexes were compared. The absorption and fluorescence emission spectra indicated that the linker-peptides mediate interaction of phycobiliproteins and prompt energy transfer. The energy transfer in the conjugates was detected by fluorescence emission spectra and confirmed by the addition of dithiothreitol. The conjugates may be used as models for studying the energy transfer mechanism in phycobilisomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007035207721
Permalink