Publication Date:
2016-04-30
Description:
Li et al (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods' reliability to visualize specimen features at sub-100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sahl, Steffen J -- Balzarotti, Francisco -- Keller-Findeisen, Jan -- Leutenegger, Marcel -- Westphal, Volker -- Egner, Alexander -- Lavoie-Cardinal, Flavie -- Chmyrov, Andriy -- Grotjohann, Tim -- Jakobs, Stefan -- New York, N.Y. -- Science. 2016 Apr 29;352(6285):527. doi: 10.1126/science.aad7983. Epub 2016 Apr 28.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany. ssahl@mpibpc.mpg.de fbalzar@mpibpc.mpg.de sjakobs@mpibpc.mpg.de. ; Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany. ; Laser Laboratory Gottingen, Hans-Adolf-Krebs-Weg 1, 37077 Gottingen, Germany. ; Institut Universitaire en Sante Mentale de Quebec, Cellular and Molecular Neuroscience Research Axis, 2601 Chemin de la Canardiere, Quebec, G1J 2G3, Canada. ; Helmholtz Zentrum Munchen, Institute of Biological and Medical Imaging, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany. Technische Universitat Munchen, Chair for Biological Imaging, Ismaningerstrasse 22, 81675 Munchen, Germany. ; Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany. University of Gottingen Medical Faculty, Department of Neurology, Robert-Koch-Str. 40, 37075 Gottingen, Germany. ssahl@mpibpc.mpg.de fbalzar@mpibpc.mpg.de sjakobs@mpibpc.mpg.de.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/27126030" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cytoskeleton/*ultrastructure
;
*Endocytosis
;
Imaging, Three-Dimensional/*methods
;
Microscopy, Fluorescence/*methods
;
Organelles/*ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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