ISSN:
1040-0397
Keywords:
Sol-gel
;
Osmium polymer
;
Enzyme inhibition
;
Amperometry
;
Cyanide determination
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An amperometric sol-gel-based enzyme inhibition electrode for the detection of low levels of cyanice was constructed by immobilizing horseradish peroxidase (HRP) and an osmium redox polymer ([Os(bpy)2(PVP)10Cl]Cl; abbreviated Os-polymer) as mediator. Upon addition of hydrogen peroxide to the solution, a bioelectrocatalytic reduction wave was observed, which was diffusion controlled. This reduction current is subsequently inhibited by cyanide. The steady-state hydrogen peroxide catalytic reduction current reached a plateau at 150 mV (vs. Ag/AgCl) and cyanide could be determined amperometrically with good sensitivity at 0mV (vs. Ag/AgCl). The concentration range of linear response, apparent inhibition constant (K), and detection limit were 0.004-0.04 mM, 0.0637 mM, and 0.5 μM, respectively. Sodium azide, potassium thiocyanide and thiourea were all found not to interfere at this potential. The biosensor was found to be stable for over 1 week on storage at room temperature.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140091416
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