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  • 1
    Electronic Resource
    Electronic Resource
    Hordeum chilense repetitive sequences. Genome characterization using biotinylated probes (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 24-32 
    Details
    ISSN: 1432-2242
    Keywords: Hordeum chilense ; Wheat ; Repeated sequences ; Molecular probes ; Biotin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A library of random DNA fragment clones of wild barley Hordeum chilense was screened for clones of repeated nucleotide sequences. Five clones were isolated that gave a stronger hybridization signal in colony and dot blot hybridization with total H. chilense DNA in comparison to Triticum aestivum DNA. Clones labelled with biotinylated nucleotides were used as probes to investigate the repeated sequences organization in the H. chilense genome. Tandemly arranged and interspersed sequences have been found, together with homology differences with related sequences present in T. Aestivum, which could allow the differentiation of H. chilense DNA when it is present in wheat. We show that biotin can replace the use of 32P in preparing repeated sequence probes for Southern and DNA dot blot analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224011
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 781-787 
    Details
    ISSN: 1432-2242
    Keywords: Glutenin ; Gliadin ; Fl monosomic analysis ; Chromosomal assignment ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new methodology to determine the chromosomal location and allelic differences of endosperm proteins in common wheat cultivars by analysis of monosomic intervarietal hybrids is reported. Endosperm proteins from the common wheats Chinese Spring, Capelle Desprez, Holdfast and Pane 247 are studied using monosomic F1 for the chromosomes of homoeologous groups 1 and 6. The proteins were fractionated by two electrophoretical techniques, SDS- and A-PAGE. The use of monosomic offers a remarkable advantage over the utilization of intervarietal substitution lines, because it permits a quicker characterization and earlier evaluation of basic material to be used in breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303526
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  • 3
    Electronic Resource
    Electronic Resource
    Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 933-940 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Two-dimensional electrophoresis ; F1 monosomic analysis ; chromosomal assignment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273684
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic mapping between Gli-B1 locus and a telomeric C-heterochromatin band in wheat (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 791-794 
    Details
    ISSN: 1432-2242
    Keywords: Genetic mapping ; Gliadin ; C-heterochromatin ; Triticum ; Polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using C-banding it has been possible to prove that the bread wheat varieties ‘Holdfast’ and ‘CapelleDesprez’ shows an intense band of telomeric heterochromatin on the short arm of chromosome 1B, while the variety ‘Pané-247’ presents a very thin band. Gliadin study using pH-acid electrophoresis revealed the existence of differences in the Gli-B1 locus in the three varieties. Analysis of the progeny of the (P x H) x CD hybrid revealed recombination between the heterochromatin C-band and locus Gli-B1, and allowed the genetic distance between the two markers to be calculated as 6.55±3.16 cMorgan. This is the first time the genetic distance from a locus to the chromosome telomere has been directly obtained in wheat. The heterochromatin C-band studied here gives us a cytological marker on chromosome 1B that can be used as a reference point in the localization of other genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224193
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  • 5
    Electronic Resource
    Electronic Resource
    Organization of repeated sequences in species of the genus Avena (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 602-607 
    Details
    ISSN: 1432-2242
    Keywords: Repetitive sequences ; Avena ; Oats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four repetitive sequences from Avena murphyi have been isolated and their genome organization studied in different species of the genus Avena. A tandem sequence array was found for the Avena species that contain the C genome. Three other dispersed sequences present in the A and C genomes were arranged in a genomespecific manner. The fact that no major differences in the hybridization patterns were found between species with the same basic genome is consistent with the current taxonomy of Avena species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226904
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  • 6
    Electronic Resource
    Electronic Resource
    A molecular linkage map of rye (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1112-1118 
    Details
    ISSN: 1432-2242
    Keywords: Key words Genetic map ; RFLP ; RAPD ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map of six chromosomes of rye, (all of the rye chromosomes except for 2R), was constructed using 77 RFLP and 12 RAPD markers. The map was developed using an F2 population of 54 plants from a cross between two inbred lines. A rye genomic library was constructed as a source of clones for RFLP mapping. Comparisons were made between the rye map and other rye and wheat maps by including additional probes previously mapped in those species. These comparisons allowed (1) chromosome arm orientation to the linkage groups to be given, (2) the corroboration of several evolutionary translocations between rye chromosomes and homoeologous chromosomes of wheat; (3) an increase in the number of available markers for target regions of rye that show colinearity with wheat. Inconsistencies in the location of markers between the wheat and rye maps were mostly detected by multi-copy probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050343
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  • 7
    Electronic Resource
    Electronic Resource
    A molecular linkage map of rye (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1112-1118 
    Details
    ISSN: 1432-2242
    Keywords: Genetic map ; RFLP ; RAPD ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map of six chromosomes of rye, (all of the rye chromosomes except for 2R), was constructed using 77 RFLP and 12 RAPD markers. The map was developed using an F2 population of 54 plants from a cross between two inbred lines. A rye genomic library was constructed as a source of clones for RFLP mapping. Comparisons were made between the rye map and other rye and wheat maps by including additional probes previously mapped in those species. These comparisons allowed (1) chromosome arm orientation to the linkage groups to be given, (2) the corroboration of several evolutionary translocations between rye chromosomes and homoeologous chromosomes of wheat; (3) an increase in the number of available markers for target regions of rye that show colinearity with wheat. Inconsistencies in the location of markers between the wheat and rye maps were mostly detected by multi-copy probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230133
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