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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 56 (1988), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A phototrophic nonsulfur bacterium, strain FR2, was capable of dark fermentative growth on fructose plus bicarbonate with a doubling time of 4 h. Formate, acetate, succinate, and lactate were formed as major fermentation products. A fumarate reduction system might function to maintain redox balance during fermentative growth of this bacterium.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 205 (2001), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A new denitrifying chemoorganotrophic bacterium capable of aerobic and anaerobic degradation of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) was isolated from activated sludge. A phylogenetic analysis based on 16S rDNA sequences indicated that the new isolate is a member of the β subclass of the Proteobacteria and represents a distinct line of descent within the family Comamonadaceae. During denitrifying growth with 3-hydroxybutyrate, PHB, or PHBV as the sole carbon source, the isolate reduced nitrate to N2 without appreciable accumulation of nitrite and nitrous oxide as intermediate products. Kinetic analyses of the denitrification with different grades of PHBV indicated that approximately 0.7 g of PHBV was required to reduce 1 g of NO3−. A high denitrification rate (19 mg N-NO3− removed h−1 g−1 dry wt of cells) was found with PHBV as the electron donor.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 58 (1989), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Several strains of Gram-negative aerobic chemoheterotrophic bacteria which produced rhodoquinone were isolated from activated sludge. All isolates contained a rhodoquinone with eight isoprene units as a major component in addition to the corresponding homologue of ubiquinone. The isolates seemed to make up a single taxon because of their homogeneity in phenotypic and chemotaxonomic properties, but could not be allocated to any known taxa of Gram-negative bacteria.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 132 (1995), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet this level was only 81%. Distance matrix tree analysis suggested that A. capsulatum belongs to a unique lineage deeply branching from the Chlamydia-Planctomyces group or from the Gram-positive line.
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  • 5
    ISSN: 1432-1432
    Keywords: Key words: Reaction center — Purple bacteria — Photosynthesis — Gene transfer — Evolution — Phylogenetic tree — Nucleotide sequence — Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Phylogenetic trees were drawn and analyzed based on the nucleotide sequences of the 1.5-kb gene fragment coding for the L and M subunits of the photochemical reaction center of various purple photosynthetic bacteria. These trees are mostly consistent with phylogenetic trees based on 16S rRNA and soluble cytochrome c, but differ in some significant details. This inconsistency implies horizontal transfer of the genes that code for the photosynthetic apparatus in purple bacteria. Possibilities of similar transfers of photosynthesis genes during the evolution of photosynthesis are discussed especially for the establishment of oxygenic photosynthesis.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 150 (1988), S. 56-60 
    ISSN: 1432-072X
    Keywords: Fumarate reduction ; Quinones ; Rhodospirillaceae ; Phototrophic bacteria ; Evolution ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nineteen established and one undesignated species of the Rhodospirillaceae were examined for fumarate reduction in connection with their quinone systems. The fumarate reductase activity with reduced methyl viologen (MVH) or FMNH2 as electron donor was found in membrane (chromatophore) preparations from phototrophically grown cells of all species containing menaquinone (MK) and/or rhodoquinone. The species having ubiquinone as the sole quinone contained no fumarate reductase activity, except some Rhodobacter species showing the FMNH2-dependent activity. The MVH-fumarate reductase activity of the MK-type species was not inhibited by Triton X-100 or acetone treatment, suggesting the presence of a fumarate reductase reacting directly with MVH, while such an enzyme was absent in the MK-lacking strains, with few exceptions. The FMNH2-fumarate reduction system was abolished by a detergent or acetone extraction in all bacteria but differed much among species with different quinone types as to the response to respiratory inhibitors. These differences in fumarate-reducing properties and quinone systems among the phototrophic bacteria are discussed from evolutionary and taxonomic viewpoints.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 151 (1989), S. 378-379 
    ISSN: 1432-072X
    Keywords: Heliobacterium chlorum ; Quinones ; Menaquinone ; Photosynthetic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thin-layer chromatography, ultraviolet spectrophotometry, high-performance liquid chromatography, and mass spectroscopy revealed that Heliobacterium chlorum contained menaquinone as the sole quinone with an average amount of 0.35 μmol per g dry weight of cells. A menaquinone homologue with nine isoprene units occurred as the major component.
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  • 8
    ISSN: 1432-072X
    Keywords: Rhodoferax fermentans ; Phototrophic bacteria ; Purple nonsulfur bacteria ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Strains of the phototrophic bacteria previously referred to as the “rhodocyclus gelatinosus-like (RGL)” group were taxonomically studied in comparison with Rhodocyclus species. Cells of the RGL strains were curved rods and motile by means of polar flagella. They contained bacteriochlorophyll a and carotenoids of the spheroidene series. The intracytoplasmic membrane system was absent. Photoorganotropho with various organic compounds as carbon sources was the preferred mode of growth. Aerobic growth at full atmospheric oxygen tension and fermentative growth under anaerobic-dark conditions were also possible. The major cellular fatty acids were palmitoleic acid and palmitic acid, and 3-hydroxylated fatty acids with octanoic acid predominating were also found. Both ubiquinone-8 and rhodoquinone-8 occurred as major quinones. The mol% guanine plus cytosine of the DNAs varied between 59.8 and 60.3. DNA-DNA hybridization studies showed that the RGL strains were highly related to each other but exhibited low levels of the homology to Rhodocyclus species. These data allow the establishment of the RGL group as a new taxon of the purple nonsulfur bacteria, for which the name Rhodoferax fermentans gen. nov., sp. nov. is proposed.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 61 (1992), S. 231-236 
    ISSN: 1572-9699
    Keywords: Zoogloea ; fatty acids ; quinones ; chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract NineZoogloea strains including the type strain ofZ. ramigera (IAM 12136=ATCC 19544=N.C. Dondero 106) and newly isolated strains were investigated for isoprenoid quinone composition and whole-cell fatty acid profiles. Seven of the tested strains, having phenotypic properties typical ofZoogloea, were characterized by their production of both ubiquinone-8 and rhodoquinone-8 as major quinones, whereas the remaining two strains,Z. ramigera IAM 12669 (=K. Crabtree I-16-M) and IAM 12670 (=P.R. Dugan 115), formed ubiquinone-10 and ubiquinone-8, respectively, as the sole quinone. All rhodoquinone-producing strains contained palmitoleic acid and 3-hydroxy-decanoic acid as the major components of nonpolar and hydroxylated fatty acids, respectively. Marked differences were noted in the fatty acid composition between the strains with and without rhodoquinones. The chemotaxonomic data suggested that the rhodoquinonelacking strains should be excluded from the genusZoogloea. Since there have been no reliable taxonomic tools forZoogloea, rhodoquinone analysis may provide a new criterion of great promise for identifyingZoogloea strains.
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  • 10
    ISSN: 1573-5117
    Keywords: vertical distribution ; total bacterial number ; bacterial biomass ; Caulobacter ; phototrophic bacteria ; Antarctic lake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vertical distribution of bacteria in Lake Vanda, an Antarctic meromictic lake, was examined by the acridine orange epifluorescence direct count method. Total bacteria were 104–105 cells · ml−1 in the water at 55 m depth and above, and increased drastically to 107 cells · ml−1 in the bottom water. Filamentous or long rodshaped bacteria occurred at a high frequency in the upper layers, but in the bottom layers most bacteria were coccoidal or short rods. Mean bacterial cell volume in water of between 10 m and 60 m deep was fairly large compared with common bacterial populations in seawater and lake water. Aerobic heterotrophic bacteria were recovered from the water of a depth of 30 m and above, and were assumed to belong to Caulobacter. Viable heterotrophic bacteria were not recovered from the high salinity deep water by media prepared with the same deep water. Phototrophic purple non-sulphur bacteria were isolated by enrichment cultures from water at 55 m depth.
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