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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 248 (1975), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Mink ; Mustela vison ; anal sac ; organosulfur compounds ; secretory granule ; glycoprotein ; apocrine ; sebaceous ; volatile fatty acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sulfur-rich anal sac secretion of the mink,Mustela vison, consisted of immiscible lipid (1.7% sulfur) and aqueous (0.7% sulfur) phases. Light and electron microscopy revealed secretory tissue of two types, sebaceous (holocrine) and apocrine. A major input of sulfur into the sac appeared to be associated with glycoprotein granules present in the apical portions of the apocrine cells as X-ray energy probe microanalysis showed these to contain relatively high levels of sulfur. The lipid of the secretion, presumed to be largely of sebaceous origin, consisted mainly of wax monoesters, while the aqueous phase contained volatile fatty acids, ammonia, and amines, including putrescine (1,4-diaminobutane). The identity of the major headspace volatiles was confirmed by NMR, MS, and Raney nickel desulfuration as being 2,2-dimethylthiacyclobutane and 3,3-dimethyl-1, 2-dithiacyclopentane. These compounds were not detected by GC-MS in the headspace volatiles of the anal sac secretions of eight other mustelid species examined. Other sulfur compounds detected included isomeric dimethylthiacyclobutanes, a number of disulfides and 3-methyl-but-3-enyl methyl sulfide (isopentenyl methyl sulfide). The significance of these findings is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Neurosecretion ; Neurophysin ; Neurohypophysia hormones ; Electron microscopy ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron-microscope autoradiographs have been prepared from the neural lobes of the pituitary glands of rats which had received intracisternal injections of [35S] cysteine at various times before gland removal. The rate of appearance and disappearance of autoradiographically demonstrable radioactivity in the neural lobe closely paralleled that previously determined, biochemically, for radioactive hormones and neurophysins. Radioactivity was appreciably associated with the undilated parts of neurosecretory axons only during the first few hours after injection of the label. The axonal dilations were subdivided into those in which small vesicles could be seen (“endings”) and those in which no small vesicles could be seen (“swellings”). Radioactivity appeared first in “endings” and then in progressively larger and larger profiles of “swellings”. It appeared that newly arrived granules were found close to the limiting membrane of the nerve swelling and that as time progressed they moved deeper and deeper into the swelling. On the basis of the results, suggestions were made for an anatomical explanation of the readily-releasable pool of hormone which has been demonstrated pharmacologically.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 109 (1970), S. 297-305 
    ISSN: 1432-0878
    Keywords: Bladder ; Bufo marinus ; Transport ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The appearance of the mucosal cell layer of the isolated urinary bladder of the toadBufo marinus, has been examined using stereoscopic and conventional transmission electron microscopy. Three cell types can be identified in surface view, these are granular cells, mitochondriarich cells and goblet cells. Cell boundaries between granular cells are clearly defined by membranous folds along their margins. Although no changes are seen in the stereoscopic electron micrographs when the granular cells are made permeable to water by vasopressin, the changes observed on transmission electron micrographs include swelling of cell bodies and nuclei, filling of intercellular channels with water, and the appearance beneath the mucosal cell membrane surface of electron dense granules. Differences between the appearance of the bladder mucosal cells by the two methods of electron microscopical examination are due largely to water loss when the tissue is freeze dried prior to stereoscopic examination.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 96 (1978), S. 361-367 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mineral deposits have been found in the cell walls of two newly described green algae,Siderocelis minor Crawford andScenedesmus granulatus West et West f.salina Crawford. A morphological account using transmission electron microscopy coupled with X-ray micro-analysis has shown that the deposits consist chiefly of iron and manganese though other elements were also found. Several possible explanations have been suggested for the variation in iron/manganese ratio—the ecological conditions pertaining in the natural habitat being considered particularly relevant.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 11 (1979), S. 137-143 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis The nucleus pulposus of cat intervertebral disc was examined after staining glycosaminoglycans with Alcian Blue and the results correlated with TEM X-ray probe microanalysis. In unstained sections a difference in copper levels between tissues and resin was detected. In tissue stained with Alcian blue before embedding, the copper levels were slightly increased and the morphological appearance of the intercellular material was amorphous. In sections restained after cutting, the relative levels of copper in the resin were considerably increased and tissue levels were significantly higher than in the resin. Moreover, the morphology of the intercellular material was altered from a rather amorphous material to a network. Sulphur levels behaved in similar manner to copper levels but any correlation between the elements was due to factors unrelated to glycosaminoglycan staining and probably resulted from contaminating sulphur.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 1970-09-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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