Publication Date:
1990-11-16
Description:
Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a "CpG island archipelago," were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bonetta, L -- Kuehn, S E -- Huang, A -- Law, D J -- Kalikin, L M -- Koi, M -- Reeve, A E -- Brownstein, B H -- Yeger, H -- Williams, B R -- CA48932/CA/NCI NIH HHS/ -- T32DK07458/DK/NIDDK NIH HHS/ -- T32GM07315/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1990 Nov 16;250(4983):994-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2173146" target="_blank"〉PubMed〈/a〉
Keywords:
*Chromosome Mapping
;
Chromosome Walking
;
*Chromosomes, Human, Pair 11
;
DNA Probes
;
*Dinucleoside Phosphates
;
Genes, Wilms Tumor/*genetics
;
Humans
;
Transcription, Genetic
;
Wilms Tumor/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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