Publication Date:
2010-05-21
Description:
Commensal bacteria are known to inhibit pathogen colonization; however, complex host-microbe and microbe-microbe interactions have made it difficult to gain a detailed understanding of the mechanisms involved in the inhibition of colonization. Here we show that the serine protease Esp secreted by a subset of Staphylococcus epidermidis, a commensal bacterium, inhibits biofilm formation and nasal colonization by Staphylococcus aureus, a human pathogen. Epidemiological studies have demonstrated that the presence of Esp-secreting S. epidermidis in the nasal cavities of human volunteers correlates with the absence of S. aureus. Purified Esp inhibits biofilm formation and destroys pre-existing S. aureus biofilms. Furthermore, Esp enhances the susceptibility of S. aureus in biofilms to immune system components. In vivo studies have shown that Esp-secreting S. epidermidis eliminates S. aureus nasal colonization. These findings indicate that Esp hinders S. aureus colonization in vivo through a novel mechanism of bacterial interference, which could lead to the development of novel therapeutics to prevent S. aureus colonization and infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Iwase, Tadayuki -- Uehara, Yoshio -- Shinji, Hitomi -- Tajima, Akiko -- Seo, Hiromi -- Takada, Koji -- Agata, Toshihiko -- Mizunoe, Yoshimitsu -- England -- Nature. 2010 May 20;465(7296):346-9. doi: 10.1038/nature09074.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bacteriology, The Jikei University School of Medicine, Tokyo, 105-8461 Japan. iwase.tadayuki@jikei.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20485435" target="_blank"〉PubMed〈/a〉
Keywords:
Bacterial Proteins/chemistry/isolation & purification/*metabolism/pharmacology
;
Biofilms/*growth & development
;
Female
;
Humans
;
Male
;
Nose/*microbiology
;
Odds Ratio
;
Serine Proteases/chemistry/deficiency/isolation & purification/*metabolism
;
Staphylococcal Infections/microbiology/prevention & control/therapy
;
Staphylococcus aureus/*growth & development/immunology
;
Staphylococcus epidermidis/*enzymology/genetics/*physiology
;
Superinfection/immunology/microbiology/prevention & control/therapy
;
Young Adult
;
beta-Defensins/immunology/pharmacology
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink