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  • 1
    Electronic Resource
    Electronic Resource
    Medium Hydration Effects on the Growth of Trichoderma Viride (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 58 (1993), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydration of gelled substrates was examined in relation to growth of the filamentous fungus Trichoderma viride. An experimental plan was designed to discriminate between the effects of thermodynamic (aw) and kinetic parameters (water content) on fungal growth. At a set thermodynamic level of water (aw), the decrease in water content caused by adding silica gel to the medium proved limiting to fungal growth. This limitation was attributed to diffusion as shown by experimental determination of rotational and translational diffusivities of glucose in such media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1993.tb06180.x
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  • 2
    Electronic Resource
    Electronic Resource
    High-performance liquid chromatography of lonidamine in human plasma and urine
    Amsterdam : Elsevier
    Journal of Chromatography B: Biomedical Sciences and Applications 277 (1983), S. 427-432 
    Details
    ISSN: 0378-4347
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)84871-9
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  • 3
    Electronic Resource
    Electronic Resource
    Estrogen fluoroimmunoassay with a fluorimeter designed for low-intensity light detection
    Amsterdam : Elsevier
    Analytica Chimica Acta 227 (1989), S. 129-134 
    Details
    ISSN: 0003-2670
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)82651-3
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of the sugar-beet pulp water activity on the solid-state culture of Trichoderma viride TS (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 537-541 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The growth and the sporulation of Trichoderma viride TS in relation to water activity (a w) of sugar-beet pulp medium was studied. It was found that the maximum growth, monitored by protein production, substrate utilization and pH alteration, appeared at a w=0.990–0.992. Optimal water activity of the medium for sporogenesis was 0.980. It was observed that both physiological phenomena appeared in narrow ranges of water activity which caused the rigorous a w control in solid-state fermentation to be postulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253028
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of water activity on production and activity of Rhizopus oligosporus polysaccharidases (1992)
    Springer
    Applied microbiology and biotechnology 37 (1992), S. 420-425 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary During tempeh fermentation, Rhizopus oligosporus produced polysaccharidases to degrade soya bean cell walls; the maximum activity for all polysaccharidases tested occurred 20–30 h after inoculation. R. oligosporus was also grown in a soya bean extract model medium to which glycerol was added to control water activity (a w). The overall activities of the major enzymes produced by the fungus, polygalacturonase, endocellulase and xylanase, appeared to be strongly influenced by a w. The production of enzymes as well as their specific activities were affected by a w. The optimum a w for polygalacturonase and xylanase activity coincided with that for mycelial growth, namely 0.99–1.00. In contrast, the optimum a w for (endo)cellulase was 0.98, at which mycelial growth was significantly reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180961
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  • 6
    Electronic Resource
    Electronic Resource
    Water activity and water content: comparative effects on the growth of Penicillium roqueforti on solid substrate (1988)
    Springer
    Applied microbiology and biotechnology 27 (1988), S. 389-392 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary This work was intended to compare simultaneously the effects of water content and of water activity values of cellulose substrate on the growth of a filamentous fungus Penicillium roqueforti. Four water activity values (0.94, 0.96, 0.97, 0.99) and four water content values: 0.4, 0.6, 0.8, 1 (g/g dry matter) have been tested in a cross experiment. The effect of water activity has been shown to be highly significant while the water content level did not significantly modify the development of the fungus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251774
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  • 7
    Electronic Resource
    Electronic Resource
    Yeast viability related to water potential variation: influence of the transient phase (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 617-622 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The decrease rate of the water potential was found to have a great effect on yeasts submitted to hypertonic shifts. The application of slow and linear decreases of the water potential of the medium to cells of Saccharomyces cerevisiae demonstrated that the cells could survive (90 to 100% of viability) very low levels of water potential (Ψ=−101 MPa). Resistance of the cells was examined through viability measurements and cell volume changes. The kinetics of cell volume variation were measured continuously using an on-line image analysis system coupled to a microscope. No biological response of the cells occurred, due to the lack of a usable carbon-energy source in the medium. The viability level was found to be a function of the water exit flow rate from the cells. The denaturation of the membrane structure was assumed to be involved in such phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173929
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  • 8
    Electronic Resource
    Electronic Resource
    Yeast viability related to water potential variation: influence of the transient phase (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 617-622 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The decrease rate of the water potential was found to have a great effect on yeasts submitted to hypertonic shifts. The application of slow and linear decreases of the water potential of the medium to cells of Saccharomyces cerevisiae demonstrated that the cells could survive (90 to 100% of viability) very low levels of water potential (Ψ= –101 MPa). Resistance of the cells was examined through viability measurements and cell volume changes. The kinetics of cell volume variation were measured continuously using an on-line image analysis system coupled to a microscope. No biological response of the cells occurred, due to the lack of a usable carbon-energy source in the medium. The viability level was found to be a function of the water exit flow rate from the cells. The denaturation of the membrane structure was assumed to be involved in such phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050302
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  • 9
    Electronic Resource
    Electronic Resource
    Escherichia coli and Lactobacillus plantarum responses to osmotic stress (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 704-709 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of −5.6 MPa and −11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was only observed when the solute was NaCl. The cell mortality during osmotic shocks induced by NaCl cannot be explained by a critical volume decrease or by the intensity of the water flow across the cell membrane. When the osmotic stress is realized with NaCl as the solute, in a medium in which osmoregulation cannot take place, the application of a slow decrease in water potential resulted in the significant maintenance of cell viability (about 70–90%) with regard to the corresponding viability observed after a sudden step change to same final water potential (14–40%). This viability difference can be explained by the existence of a critical internal free Na+ concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051354
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  • 10
    Electronic Resource
    Electronic Resource
    A simple particle image velocimetry instrument for low-velocity flows (2000)
    Springer
    Experiments in fluids 28 (2000), S. 195-196 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  A P.I.V. instrument using two synchronized CCD video cameras is described. The same field of view is imaged onto each CCD array with an image splitter and one imaging lens. The delay between two exposures can be adjusted from 0.5 to 20 ms depending on the flow velocity to be measured.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050025
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