ISSN:
1432-0983
Keywords:
Saccharomyces cerevisiae
;
Mitochondrial DNA
;
Method of extraction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A rapid method for the extraction of yeast mitochondrial DNA (mtDNA) is described. In comparison with previous methods, it simplifies several steps, does not require either the isolation of mitochondria or phenol treatment and is less time consuming. This protocol gives a high yield of pure mtDNA (50–120 μg from a 100-ml culture), which can be directly used in various molecular applications: restriction enzyme digestion, electrophoresis, blotting, labeling, cloning and sequencing.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00435511
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