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  • 1
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A modification of the ‘cold plaque’ screening technique (Hodge et al., Plant Journal1992, 2, 257–260) was used to screen a cDNA library constructed from drought-stressed leaf tissue of the desiccation tolerant (‘resurrection’) grass Sporobolus stapfianus. This technique allowed a large number of clones representing genes expressed at low abundance to be isolated. An examination of expression profiles revealed that several of these genes are induced in desiccation-tolerant tissue experiencing severe drought stress. Further characterization indicated that the gene products encoded include an eIF1 protein translation initiation factor and a glycine- and proline-rich protein which have not previously been associated with drought stress. In addition, genes encoding a serine/threonine phosphatase type 2C, a tonoplast-intrinsic protein (TIP) and an early light-inducible protein (ELIP) were isolated. A number of these genes are expressed differentially in desiccation-tolerant and desiccation-sensitive tissues, suggesting that they may be associated with the desiccation tolerance response of S. stapfianus. The results indicate that there may be unique gene regulation processes occurring during induction of desiccation tolerance in resurrection plants which allow different drought-responsive genes to be selectively expressed at successive levels of water loss.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 38 (1976), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The relationship between leaf resistance to water vapour diffusion and each of the factors leaf water potential, light intensity and leaf temperature was determined for leaves on seedling apple trees (Malus sylvestris Mill. cv. Granny Smith) in the laboratory. Leaf cuticular resistance was also determined and transpiration was measured on attached leaves for a range of conditions.Leaf resistance was shown to be independent of water potential until potential fell below — 19 bars after which leaf resistance increased rapidly. Exposure of leaves to CO2-free air extended the range for which resistance was independent of water potential to — 30 bars.The light requirement for minimum leaf resistance was 10 to 20 W m−2 and at light intensities exceeding these, leaf resistance was unaffected by light intensity.Optimum leaf temperature for minimum diffusion resistance was 23 ± 2°C. The rate of change measured in leaf resistance in leaves given a sudden change in leaf temperature increased as the magnitude of the temperature change increased. For a sudden change of 1°C in leaf temperature, diffusion resistance changed at a rate of 0.01 s cm−1 min−1 whilst for a 9°C leaf temperature change, diffusion resistance changed at a rate of 0.1 s cm−1 min−1.Cuticular resistance of these leaves was 125 s cm−1 which is very high compared with resistances for open stomata of 1.5 to 4 s cm−1 and 30 to 35 s cm−1 for stomata closed in the dark.Transpiration was measured in attached apple leaves enclosed in a leaf chamber and exposed to a range of conditions of leaf temperature and ambient water vapour density. Peak transpiration of approximately 5 × 10−6 g cm−2 s−1 occurred at a vapour density gradient from the leaf to the air of 12 to 14 g m−3 after which transpiration declined due presumably to increased stomatal resistance. Leaves in CO2-free air attained a peak transpiration of 11 × 10−6 g cm−2 s−1 due to lower values of leaf resistance in CO2 free air. Transpiration then declined in these leaves due to development of an internal leaf resistance (of up to 2 s cm−1). The internal resistance was masked in leaves at normal CO2 concentrations by the increase in stomatal resistance.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 48 (1980), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Transpiration was measured in apple leaves (Malus sylvestris Mill.) which were enclosed in a leaf chamber and subjected to rapid changes in leaf temperature. Fluctuations in leaf temperature produced parallel fluctuations in transpiration. The change in transpiration rate with change in temperature was found to be less than the theoretical value calculated from the change in water vapour density gradient from leaf to air. The results suggest the presence of a small and rapidly varying resistance to water vapour loss from the leaf. The magnitude of this additional resistance increased to a maximum value of approximately 1.5 s cm-1 as the magnitude of the temperature change increased to a maximum of approximately 12°C.
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  • 4
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A change occurs in the relationship between xylem water potential (Ψx) measured with the pressure chamber and leaf water potential ((Ψw)) when a period of post-excision water loss is allowed before measurement of (Ψx) and (Ψx). When this occurs, water stress is over-estimated by the pressure chamber measurement. Over the same period of water loss, cavitation vibrations have been detected acoustically in excised leaves. It is suggested that the measurement of (Ψx) is affected by emptying of some of the xylem vessels due to cavitation. This would require that additional pressure be applied to a leaf in the pressure chamber in order to measure (Ψx).
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 74 (1987), S. 133-136 
    ISSN: 1432-1939
    Keywords: Desiccation tolerance ; Ferns ; Grass ; Tripogon ; Veloziaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Numerous Velloziaceae species concentrated in Minas Gerais, Brazil, constitute most of the desiccation tolerant flora of S. America. In the grasses, two Microchloa spp and Tripogon spicatus are desiccation tolerant. Though widespread, they are low growing and appear unimportant. With the exception of Anemia, desiccation tolerant ferns in dry land areas are related to the “resurrection” fern flora reported for N. America. The likely possibility of a desiccation tolerant flora of epiphytic ferns in rain forest areas was not investigated.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 70 (1986), S. 118-120 
    ISSN: 1432-1939
    Keywords: Eragrostis ; Kyllinga ; Resurrection Grasses ; Sporobolus ; Tripogon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Only a very small proportion of angiospermae have foliage which can recover (within 24 h) from full airdryness. The number of such plants was extended by five grass species, in the genera Eragrostis, Sporobolus and Tripagon, and by two sedges, Kyllinga spp. The basal leaf meristematic zones in most species survived equilibration to air of 0 to 5% RH, whereas mature leaf tissue in most survived 5 to 30% RH.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 78 (1989), S. 407-410 
    ISSN: 1432-1939
    Keywords: ATP ; ADP ; Desiccation tolerance ; Rehydration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary All species of desiccation tolerant angiosperms studied conserved some ATP in air-dry viable leaves. Otherwise there was no uniformity of response to drying: ATP content remained high in some species, but fell markedly in the others. ATP levels stayed high in Boea hygroscopica and Borya constricta until relative water content fell below 30%. ATP content recovered in the first 2 h of rehydration in Boea, but recovery was gradual in Borya, taking more than 16 h.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 71 (1986), S. 159-160 
    ISSN: 1432-1939
    Keywords: Resurrection plants ; Grasses ; India ; Grazing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A search for desiccation tolerant grasses in India revealed nine grass species (in the genera Eragrostiella, Oropetium and Tripogon) whose ability to recover from airdryness had not previously been reported. Dry leaves of these species recover uninjured within 24 h of plants receiving water. Desiccation tolerance limits were 0%–11% RH (at 28°C). Some species were relatively tall and vigorous and may be useful for grazing purposes in arid areas.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Oecologia 78 (1989), S. 559-564 
    ISSN: 1432-1939
    Keywords: Drought tolerance ; pH tolerance ; Poaceae ; Resurrection grass ; Salt tolerance ; Sporobolus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dry matter productivity under saline conditions was compared in 5 desiccation-tolerant “resurrection” grasses and one desiccation sensitive species, all in the genus Sporobolus. S. stapfianus was the most salt tolerant, requiring 215 mole NaCl m-3 to reduce shoot dry matter increments to 50% of increments in plants not treated with salt. (This was comparable to published values for the salt tolerant grass Diplachne fusca.) S. lampranthus was salt sensitive, requiring 35 mol m-3 for 50% control yields. S. festivus, S. aff. Fimbriatus, and the deisccation sensitive S.pyramidalis was moderately tolerant (150–170 mol m-3). The moderate salt resistance of S. aff. fimbriatus was attributed mainly to exclusion of NaCl by roots. Salt export through leaf surfaces was a minor factor. Half of the leaf mesophyll cells survived 50 min immersion in 200 mol NaCl m-3. Plants of S. aff. fimbriatus and S. pyramidalis tolerated a broad range of soil pH. Plants of 4 desiccation tolerant Sporobolus species survived air-dryness following 3 weeks pretreatment with salinities up to 200 mol m-3
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 72 (1985), S. 608-609 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
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