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  • 1
    Electronic Resource
    Electronic Resource
    A RAPID METHOD FOR THE DETECTION OF PSE AND DFD PORCINE MUSCLES (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Results of tests made on 253 porcine muscles showed that 1 hr after death PSE and DFD muscles contain low levels of adenosine triphosphate (ATP) and high levels of inosine monophosphate while in normal muscles high concentrations of ATP and low amounts of inosine monophosphate are observed. The deamination of the adenine moiety can be measured by the changes in the absorption ratio at 250 and 260 nm. This ratio (called R) amounts to 0.78–0.80 for pure adenine nucleotide solutions and to 1.68–1.70 for pure inosine monophosphate and inosine in aqueous solutions at neutral pH. Preparation of acidic extract of muscles containing the nucleotides and the measurement of the 250/260 nm ratio of absorbances is a fast procedure: 3–4 min after receiving the sample the R values can be calculated. Together with pH1 the R value allows a very reliable differentiation of normal, PSE and DFD muscles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1977.tb08444.x
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  • 2
    Electronic Resource
    Electronic Resource
    Food reserves of Scots pine (Pinus sylvestris L.) (1991)
    Springer
    Trees 5 (1991), S. 187-195 
    Details
    ISSN: 1432-2285
    Keywords: Food reserves ; Pinus sylvestris ; Starch ; Sugars ; Triacylglycerol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amounts of starch, soluble sugars, triacylglycerols, diacylglycerols and free fatty acids were studied in Scots pine (Pinus sylvestris L.) during an annual cycle in current-year needles and in 1-, 2- and 3-year-old needles collected shortly after bud break. Determination of the compounds was performed using specific enzymatic assays, capillary gas chromatography and thin layer chromatography. Newly emerging needles contained relatively large amounts of starch, but only trace amounts of fat. During autumn and winter, fat content rose, while starch content decreased; amounts of both these reserve materials were very high the next spring shortly before bud break and decreased again during shoot elongation. Concentration of intermediates in triacylglycerol biosynthesis (diacylglycerols and free fatty acids), were low in summer and high in winter. The same pattern was observed for fructose and glucose (the predominant soluble sugars), galactose/arabinose and raffinose/melibiose. In contrast, sucrose concentrations were highest in spring and in autumn. Mature needles of different ages collected in May showed significant differences only in their triacylglycerol and starch content. Concentration changes of reserve materials are discussed in relation to season, mobilization and translocation processes, dormancy, frost resistance and the possibility of carbohydrate-fat interconversions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227524
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  • 3
    Electronic Resource
    Electronic Resource
    Food reserves of scots pine (Pinus sylvestris L.) (1992)
    Springer
    Trees 6 (1992), S. 147-155 
    Details
    ISSN: 1432-2285
    Keywords: Pinus sylvestris ; Starch ; Sugars ; Triacylglycerols ; Xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Starch, soluble sugars, triacylglycerols, diacylglycerols and free fatty acids were measured in 30-year-old Scots pine (Pinus sylvestris L.) trees during an annual cycle in the sapwood (youngest ten xylem rings). The radial distribution of carbohydrates and lipids was studied in the trunkwood of 90 -to 150-year-old Scots pine trees collected at the end of the growing season. Determination of the compounds was performed using specific enzymatic assays, capillary gas chromatography and thin layer chromatography. The amounts of glucose, fructose, sucrose, and galactose/arabinose in the sapwood were slightly higher in winter than in summer. Raffinose/stachyose increased up to 5-fold during the cold period. At the beginning of the growing season starch amounts rose, and then decreased in summer and autumn. No concentration changes were observed in the total amounts of diacylglycerols and fatty acids throughout the year. Triacylglycerol levels were slightly higher in February than in summer and autumn. Relative frequencies of individual fatty acids were similar in all lipid fractions. Glucose, fructose, sucrose, starch and triacylglycerols disappeared almost entirely at the transition zone from sapwood to heartwood. In contrast, free fatty acids and galactose/arabinose rose in centripetal direction, and diacylglycerols remained constant across trunk cross-sections. The relative amounts of individual fatty acids changed markedly in the free fatty acid fraction and in the triacylglycerols when crossing the sapwood-heartwood boundary. Concentration changes of reserve materials are discussed in relation to season, mobilization and translocation processes, dormancy, frost resistance, and heartwood formation. The results are compared to those found in needles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202430
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  • 4
    Electronic Resource
    Electronic Resource
    Another pedigree with pure autosomal dominant spastic paraplegia (AD-FSP) from Tibet mapping to 14q11.2–q24.3 (1997)
    Springer
    Human genetics 〈Berlin〉 100 (1997), S. 620-623 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mutant in a family with autosomal-dominant spastic paresis in Northern Tibet was mapped by linkage analysis with several microsatellite markers to a gene locus at 14q11.2–q24.3, an area to which a few mutants leading to a condition with similar clinical signs have previously been mapped. The mutant observed in this pedigree probably arose de novo. Gene loci at 2p21– p24 and 15q, which have been found for other pedigrees with dominant spastic paresis, were excluded. The data in this pedigree do not contradict the hypothesis proposed by another group that there might be anticipation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050563
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  • 5
    Electronic Resource
    Electronic Resource
    A genetic study of the human low-voltage electroencephalogram (1992)
    Springer
    Human genetics 〈Berlin〉 90 (1992), S. 99-112 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The studied phenotype, the low-voltage electroencephalogram (LVEEG), is characterized by the absence of an alpha rhythm from the resting EEG. In previous studies, evidence was found for a simple autosomal-dominant mode of inheritance of the LVEEG. Such a polymorphism in brain function can be used as a research model for the stepwise elucidation of the molecular mechanism involved in those aspects of neuronal activity that are reflected in the EEG. Linkage with the variable number of tandem repeats (VNTR) marker CMM6 (D20S19) and localization of an LVEEG (EEGV1) gene on 20q have previously been reported, and genetic heterogeneity has been demonstrated. This latter result has been corroborated by studing new marker (MS214). The phenotype of the LVEEG is described here in greater detail. Its main characteristic is the absence of rhythmic alpha activity, especially in occipital leads, whereas other wave forms such as beta or theta waves may be present. Analysis of 17 new families (some of them large), together with 60 previously described nuclear families, supports the genetic hypothesis of an autosomal-dominant mode of inheritance. Problems connected with the analysis of linkage heterogeneity, exclusion mapping, and the study of multipoint linkage are discussed. A possible explanation of the localization of LVEEG in the close vicinity of another gene influencing synchronization of the normal EEG, the gene for benign neonatal epilepsie, is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210751
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  • 6
    Electronic Resource
    Electronic Resource
    High-resolution comparative hybridization to combed DNA fibers (1997)
    Springer
    Human genetics 〈Berlin〉 99 (1997), S. 374-380 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Comparative genomic hybridization (CGH) has proven to be a comprehensive new tool to detect genetic imbalances in genomic DNA. However, the resolution of this method carried out on normal human metaphase spreads is limited to low copy number gains and losses of ≥ 10 Mb. An improved resolution allowing the detection of copy number representations of single genes would strongly enhance the applicability of CGH as a diagnostic and research tool. This goal may be achieved when metaphase chromosomes are replaced by an array of target DNAs representing the genes of interest. To explore the feasibility of such a development in a model system we used cosmid MA2B3, which encompasses about 35 kb in the vicinity of exon 48 of the human dystrophin gene. Linearized cosmid fibers were attached to a glass surface and aligned in parallel by “molecular combing”. Two-color fluorescence in situ suppression hybridization was performed on these cosmid fibers with probe mixtures containing different ratios (ranging from 1:2 to 4:1) of biotin- and digoxigenin-labeled MA2B3 cosmid DNAs. For each mixture fluorescence ratios were determined for 40–50 individual combed DNA molecules. In two series comprising a total of 651 molecules the median fluorescence ratio measurements revealed a linear relationship with the chosen probe ratios. Our study demonstrates that fluorescence ratio measurements on single DNA molecules can be performed successfully.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050375
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  • 7
    Electronic Resource
    Electronic Resource
    Programs, databases, and expert systems for human geneticists — a survey (1996)
    Springer
    Human genetics 〈Berlin〉 97 (1996), S. 129-137 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We present an overview of the variety of databases and programs that offer substantial aid to medical and molecular geneticists. Databases and expert systems for genetic diseases and birth defects, programs for segregation and linkage analysis, certain DNA and protein sequence databases, and information resources in general for molecular biology are addressed. These systems cannot be used effectively without the newly developed techniques of information exchange based on international computer networks. A short introduction is given to the Internet and to European institutions and organizations that offer help with the aquisition and use of bioinformatic resources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265253
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  • 8
    Electronic Resource
    Electronic Resource
    Benign familial neonatal convulsions: confirmation of genetic heterogeneity and further evidence for a second locus on chromosome 8q (1995)
    Springer
    Human genetics 〈Berlin〉 95 (1995), S. 411-415 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The syndrome of benign familial neonatal convulsions (BFNC) is a rare, autosomal dominant form of epilepsy. It is characterized by spontanous seizures beginning within the first 6 months of life. In the majority of families linkage is to chromosome 20q markers. Based on the linkage results in one large BFNC kindred, genetic heterogeneity and existence of a second locus on chromosome 8 have been suggested. Here we report on a second BFNC family in which linkage to the EBN1 locus on chromosome 20q was excluded, confirming the genetic heterogeneity of this disorder. All affected family members experienced onset of seizures before the age of 2 months. Three BFNC subjects showed subsequent epileptic seizures after 12 months of age, showing that the risk of subsequent epilepsy is not restricted to the chromosome 20q linked BFNC families. A lod score of 0.99 was obtained with the marker D8S274, suggesting linkage to chromosome 8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208966
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  • 9
    Electronic Resource
    Electronic Resource
    Enantioselective synthesis of α-aminophosphonic acid derivatives by hydrogenation (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 10 (1998), S. 564-572 
    Details
    ISSN: 0899-0042
    Keywords: asymmetric hydrogenation ; aminophosphine phosphinites ; rhodium complexes ; dehydro aminophosphonic acids ; NMR ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Chiral α-aminophosphonic acid derivatives are efficiently synthesized by asymmetric hydrogenation of the prochiral N-acyl-α,β-dehydroaminophosphonates. PROPRAPHOS-Rh-catalysts from readily available (S)- and (R)-Propranolol proved to be suitable in the homogenous reaction affording an enantiomeric excess of 87-92% with high rate. The aminophosphonic acid derivatives and precursors were fully characterized by 1H, 13C, and 31P NMR spectroscopy. Chirality 10:564-572, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Complexes of Group 3 Metals and Lanthanides That Contain Siloxane-Bridged Bisaminopyridinato Ligands: Synthesis, Structure, and Application in the Ring-Opening Polymerization of Lactones (1999)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 1999 (1999), S. 2283-2288 
    Details
    ISSN: 1434-1948
    Keywords: N-ligands ; Yttrium ; Samarium ; Ring-opening polymerization ; Lactones ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The reaction of one equivalent of dilithiated O(SiMe2-Ap-H)2 [Ap-H = -N(2-amino-4-methylpyridine)] (1), generated in situ, with LnCl3 (Ln = Y, Sm) in THF affords O(SiMe2-Ap)2YCl(THF)2 (2) or O(SiMe2-Ap)2SmCl(THF)3 (3). In contrast, the reaction of one or two equivalents of dilithiated 1, again generated in situ, with LaBr3 in THF affords O(SiMe2-Ap)2)2LaLi(THF)3 (4). An X-ray structural analysis of 2 and 3 reveal the O(SiMe2-Ap)2- ligand to bind in a planar tetradentate manner. Equivalent Sm-N distances in 3 indicate a delocalized binding mode. Compound 2 reacts with Bu4NBH4, NaBH4 or LiCH(SiMe3)2 to give the corresponding “ate” complexes O(SiMe2-Ap)2Y(BH4)Cl(THF) Bu4N (5), O(SiMe2-Ap)2Y(BH4)2Na(THF)2 (6) and O(SiMe2-Ap)2Y(CH(TMS)2)2Li(THF)3 (7), respectively. The steric demand of the O(SiMe2-Ap)2- ligand is not large enough to stabilize monoalkyl or monoborohydride complexes. Complex 4 has been used as an initiator for the ring-opening polymerization of ε-caprolactone or δ-valerolactone. In both cases an almost linear relation between the monomer-to-initiator ratio and the molecular weight of the obtained polyester is observed. By conducting the polymerization in neat ε-caprolactone at room temperature a solid polyester block is formed after 3 min (300000 g·mol-1, Mw/Mn 2.3).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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