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  • 1
    Electronic Resource
    Electronic Resource
    Two distinct protein–protein interactions between the NIT2 and NMR regulatory proteins are required to establish nitrogen metabolite repression in Neurospora crassa (1997)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 26 (1997), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Nitrogen metabolism is a highly regulated process in Neurospora crassa. The structural genes that encode nitrogen catabolic enzymes are subject to nitrogen metabolite repression, mediated by the positive-acting NIT2 protein and by the negative-acting NMR protein. NIT2, a globally acting factor, is a member of the GATA family of regulatory proteins and has a single Cys2/Cys2 zinc finger DNA-binding domain. The negative-acting NMR protein interacts via specific protein–protein binding with two distinct regions of the NIT2 protein, a short alpha-helical motif within the NIT2 DNA-binding domain and a second motif at its carboxy terminus. Deletions of segments of NIT2 throughout most of its length result in truncated proteins, which are still functional for activating gene expression; most of these mutant NIT2 proteins still allow proper nitrogen repression of nitrate reductase synthesis. In contrast, deletions or certain amino acid substitutions within the zinc finger and the carboxy-terminal tail result in a loss of nitrogen metabolite repression. Those mutated forms of NIT2 that are insensitive to nitrogen repression have also lost one of the NIT2–NMR protein–protein interactions. These results provide compelling evidence that the specific NIT2–NMR interactions have a regulatory function and play a central role in establishing nitrogen metabolite repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1997.6041979.x
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition of Processing of the Primary Transcript of the Gene Encoding Tissue Inhibitor of Metalloproteinases (TIMP) by Antisense TIMP RNA in Mouse 3T3 Cells (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 660 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb21085.x
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  • 3
    Electronic Resource
    Electronic Resource
    Hydroxyapatite Formation and Protein Absorption on Triethyl Phosphate Modified Titanium Surface (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 26-28 (Oct. 2007), p. 797-800 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this work, triethyl phosphate (TEP) was used to bioactivating titanium. Titanium platesgrafted with TEP were immersed in a two times concentrated simulated body fluid (2SBF) toinvestigate deposition of hydoxyapatite (HA) on the surface. A phosphate buffer solution (PBS) withbovine serum albumin (BSA) was used to evaluate adsorption of protein on the grafted titaniumsurface. The morphology, component and structure of samples were examined by scanning electronicmicroscopy, attenuated total reflection Fourier transform infrared spectroscopy and X-ray diffractionrespectively. The concentration change of BSA in adsorption test was examined with theultraviolet-visible absorption spectra (UV). The analyses showed that TEP grafted onto the titaniumsurface. In 2SBF, calcium and phosphate ions deposited spontaneously onto the grafted titaniumsurface and formed a HA coating with a network-like microporous structure after being immersed for3 days. The coating consisted of HA particles with 180-265nm in thickness and 72-85nm in width.The diameter of the micropores was about 200nm. The HA coating appeared better uniformity thanthat on the modified titanium using phosphoric acid. BSA rapidly adsorbed onto the grafted titaniumsurface at first half an hour and then the adsorption quantity almost kept constant. These resultsindicate that TEP grafting is an effective approach to modify bioactivity of titanium
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/40/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.26-28.797.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    The Effect of Different Surface Modification Agents on the Dispersion of Nano-Hydroxyapatite (n-HA) Crystallites (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 284-286 (Apr. 2005), p. 55-58 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The surface of synthetic calcium hydroxyapatite (HA, Ca10(PO4)6(OH)2) was modified through combining freeze-drying, solvent replacement and surface modification methods in an effort to obtain the deaggregative n-HA crystallites. Three surface modification agents: Polyethylene glycol (PEG, Mw: 6000), poly(propyl oxide)-poly (ethyl oxide)-co-poly(propyl oxide)(Pluronic F-127, Mw: 12,000) and poly(d, l-lactide)-co-poly (ethylene glycol) (PELA, Mw: 20,000)were selected. The dispersion of the modified n-HA was characterized by sedimentation time in distilled water, acetone and dimethyl formamide (DMF). The results indicate that the three surface modification agents influence the dispersion of n-HA crystallites in various solvent based on different mechanisms. Transmission electron microscopy (TEM) observation shows that the dried and acetone-replacement powders are composed of needle-like HA crystallites isolated individually. The chosen solvents have a profound effect on the sedimentation time. In distilled water, the dispersion of as-synthesized n-HA crystallites is increased greater by F127 than by PEG. After freeze-drying, the two n-HA particles show homogeneous dispersion in acetone. The colloid stability of freeze-dried HA/PEG, solution-replaced HA/F127 and solution-replaced HA/PELA in DMF were improved significantly. The suspensions remained stable after 30 days with only a bluetransparent sol being observed
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.284-286.55.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Immobilization of Bovine Serum Albumin on Titanium through Plasma Polymerization of Allylamine and Crylic Acid (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 361-363 (Nov. 2007), p. 713-716 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In the present work, technique of plasma polymerization was used to generate amido (-NH2) and carboxyl (-COOH) on titanium surface for immobilizing bovine serum albumin (BSA).After plasma polymerization of allylamine and crylic acid, the contact angle with respect to doubledistilled water significantly increased. Surface components were detected by X-ray photoelectronspectroscopy and Fourier transform infrared reflection-absorption spectroscopy. The results showedthat BSA was successfully immobilized on the titanium surface and the amounts of BSA onspecimens were relied on the properties of thin film deposited through plasma polymerization,which was corresponding to the ultraviolet spectrophotometer result
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.713.pdf
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  • 6
    Electronic Resource
    Electronic Resource
    Calcium Phosphate Coating on Titanium Induced by Phosphating (2000)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 192-195 (Sept. 2000), p. 167-170 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/45/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.192-195.167.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Fabrication of TiO〈sub〉2〈/sub〉-Apatite Composite Coating on Titanium by a Chemical Method (2003)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 240-242 (May 2003), p. 323-326 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/46/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.240-242.323.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    Fabrication of Microporous Apatite Coating on Titanium by a Novel Chemical Method (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 284-286 (Apr. 2005), p. 231-234 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A microporous apatite coating was fabricated by a heat-oxidation and chemical routine. The morphology, composition and structure were characterized by scanning electron microscopy with X-ray energy dispersion spectroscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The components of the coating were predominantly apatite, next tri-calcium phosphate and octa-calcium phosphate. The pore size ranged from 1µm to5µm. The thickness of the coating was about 6µm ~ 10µm. The microporous coating was Ca-deficient carbonate apatite
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.284-286.231.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    The regulatory protein NIT4 that mediates nitrate induction inNeurospora crassa contains a complex tripartite activation domain with a novel leucine-rich, acidic motif (1996)
    Springer
    Current genetics 29 (1996), S. 537-548 
    Details
    ISSN: 1432-0983
    Keywords: Neurospora ; Nitrogen regulation ; NIT4 ; Transactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression ofnit-3 andnit-6, the structural genes which encode nitrate reductase and nitrite reductase inNeurospora crassa, requires the global-acting NIT2 and the pathway specific NIT4 regulatory proteins. NIT4, which consists of 1090 amino-acid residues, possesses a Cys6/Zn2 zinc cluster DNA-binding-domain. NIT4 was dissected to localize transactivation domains by fusion of various segments of NIT4 to the DNA-binding domain of GAL4 for in vivo analysis in yeast. Three separate activation subdomains, and one negative-acting region, which function in yeast were located in the carboxyl-terminal region of NIT4. The C-terminal tail of 28 amino-acid residues was identified as a minimal activation domain and consists of a novel leucine-rich, acidic region. Most deletions which removed even small segments of the NIT4 protein were found to lead to the loss of NIT4 function in vivo inN. crassa, implying that the central region of the protein which lies between the DNA-binding and activation domains is essential for function. The yeast two-hybrid system was employed to identify regions of NIT4 responsible for dimer formation. A short isoleucine-rich segment downstream from the zinc cluster, predicted to form a coiled coil, allowed dimerization in vivo; this same isoleucine-rich region also showed dimerization in vitro when examined via chemical cross linking. The enzyme nitrate reductase has been postulated to exert autogenous regulation by directly interacting with the NIT4 protein. This possible nitrate reductase-NIT4 interaction was investigated with the yeast two-hybrid system and by direct in vitro binding assays; both assays failed to identify such a protein-protein interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426958
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  • 10
    Electronic Resource
    Electronic Resource
    Nucleation and Growth of Bone-Like Apatite on Surfaces of Metals, Ceramics and Polymers in Simulated Body Fluids (2005)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 288-289 (June 2005), p. 277-280 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The biomimetic approach of mineralization in vitro is adopted to investigatesystematically the nucleation and growth of bone-like apatite on the surface of biomaterials such as bioceramics, metals and polymers, and those chemically surface-treated. The simulated environment is kept isothermic at the human body temperature of 36.5C with three kinds of simulated physiological fluids. The experimental results show that (1) inherent properties of biomaterials determine their bioactivity and the different crystalline structure of same materials results in thedifference in bioactivity; (2) the bioactivity can effectively be improved by the surface treatment of biomaterials via chemical methods and by the addition of bioactive particles in a polymer matrix; (3) the bone-like apatite, nucleated and grown in the simulated body fluid with the same ion concentrations to that of the human plasma, possesses the same composition, structure and morphology despite of matrixes; (4) the difference in bioactivity with biomaterials is indicated by the different time for bone-like apatite to nucleate and to grow on their surfaces
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/49/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.288-289.277.pdf
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