Publication Date:
2010-12-04
Description:
Asymmetric segregation of P granules during the first four divisions of the Caenorhabditis elegans embryo is a classic example of cytoplasmic partitioning of germline determinants. It is thought that asymmetric partitioning of P granule components during mitosis is essential to distinguish germline from soma. We have identified a mutant (pptr-1) in which P granules become unstable during mitosis and P granule proteins and RNAs are distributed equally to somatic and germline blastomeres. Despite symmetric partitioning of P granule components, pptr-1 mutants segregate a germline that uniquely expresses P granules during postembryonic development. pptr-1 mutants are fertile, except at high temperatures. Hence, asymmetric partitioning of maternal P granules is not essential to specify germ cell fate. Instead, it may serve to protect the nascent germline from stress.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072820/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072820/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gallo, Christopher M -- Wang, Jennifer T -- Motegi, Fumio -- Seydoux, Geraldine -- GM080042/GM/NIGMS NIH HHS/ -- HD007276/HD/NICHD NIH HHS/ -- HD037047/HD/NICHD NIH HHS/ -- R01 HD037047/HD/NICHD NIH HHS/ -- R01 HD037047-12/HD/NICHD NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2010 Dec 17;330(6011):1685-9. doi: 10.1126/science.1193697. Epub 2010 Dec 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, 725 North Wolfe Street, PCTB 706, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21127218" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blastomeres/*physiology
;
Caenorhabditis elegans/*embryology/genetics/metabolism
;
Caenorhabditis elegans Proteins/genetics/*metabolism/physiology
;
Cytoplasm/*metabolism
;
Cytoplasmic Granules/*physiology/ultrastructure
;
Embryo, Nonmammalian/physiology
;
Embryonic Development
;
Germ Cells/*physiology
;
Interphase
;
Microscopy, Confocal
;
Mitosis
;
Mutation
;
Nuclear Proteins/metabolism
;
Protein-Serine-Threonine Kinases/metabolism
;
RNA, Helminth/*metabolism
;
RNA-Binding Proteins/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Zygote/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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